摘要
利用苯酚-硫酸法和蒽酮-硫酸法对15份菜用甘薯和2份鲜食甘薯材料的叶片、叶柄和茎的多糖含量进行测定,并对2种方法进行了比较,进一步优化了甘薯茎叶多糖的提取方法,确定了最佳沸水浴时间为2 h,同时通过显色条件和方法学研究,确定了苯酚-硫酸法和蒽酮-硫酸法的最适检测波长分别为486,625 nm。结果表明,苯酚-硫酸法在稳定性和准确性上均优于蒽酮-硫酸法;采用苯酚-硫酸法对17份甘薯多糖含量测定结果为:叶片3.00%~4.69%,叶柄2.34%~4.21%,茎1.98%~3.63%;采用蒽酮-硫酸法测定结果为:叶片1.11%~2.52%,叶柄1.37%~2.85%,茎1.26%~2.69%。与前人的研究结果进行比较分析,推测蒽酮-硫酸法测定甘薯茎叶多糖更适用于提纯去除杂质后的样品。
This study aimed to establish methods for determining polysaccharide content in sweet potato and compare the possibility and difference by that of phenol-sulfuric acid method and anthrone-sulfuric acid method. The content of polysaccharides in leaf, petiole and stem of 15 vegetable-used sweet potatoes and 2 ordinary sweet potatoes were determined, which provided the theoretical basis for breeding. The extraction condition of polysaccharides from sweet potato leaf, petiole and stem was optimized, and the best boiling water bath was 2 hours. Chromogenic conditions and the methodology of phenol-sulfuric acid method and anthrone-sulfuric acid method on the determination of polysaccharides from sweet potato leaf, petiole and stem were studied. The detection wavelengths of phenol-sulfuric acid method and anthrone-sulfuric acid were 486, 625 nm, respectively. The results showed that the phenol-sulfuric acid method was better than the anthrone-sulfuric acid method of stability and accuracy. The results of polysaccharide content from sweet potato leaf, petiole and stem by phenol-sulfuric acid method were 3.00%-4.69%, 2.34%-4.21%, and 1.98%-3.63%, respectively, and that of anthrone-sulfuric acid were leaf 1.11%-2.52%, petiole 1.37%-2.85%, stem 1.26%-2.69%. Comparing with the results of previous studies, it is speculated that the anthrone-sulfuric acid method is more suitable for the purified sample on determination of polysaccharides from sweet potato.
出处
《山西农业科学》
2018年第2期182-186,206,共6页
Journal of Shanxi Agricultural Sciences
基金
四川省财政创新工程项目(2017QNJJ-021
2017QNJJ-001
2015JSCX-003
2016LWJJ-002
2016ZYPZ-005)
四川省薯类育种攻关和国家甘薯产业技术体系(CARS-10-B5)
关键词
甘薯
多糖
苯酚-硫酸法
蒽酮-硫酸法
sweet potato
polysaccharides
phenol-sulfuric acid method
anthrone-sulfuric acid method
