摘要
目的 以切断迷走神经为干预手段，探讨“通调针法”电针治疗乳腺增生（mammary gland hyperplasia, MGH）模型大鼠的作用机制。方法 取健康未孕雌性SD大鼠75只，用随机数字表法分为空白组10只、模型组13只、正常电针组13只、迷切电针组13只、假手术电针组13只、迷切不电针组13只。除空白组外均制备MGH大鼠模型，模型成功后，迷切电针组、迷切不电针组采用右侧颈部迷走神经切断术处理；假手术电针组仅暴露迷走神经不切断。各电针组均用“通调针法”电针刺激。分别于实验前后测量大鼠乳头高度，光镜观察乳腺组织形态学，电镜观察乳腺超微结构变化，流式细胞仪检测大鼠外周血中T淋巴细胞亚群CD3＋、CD4＋、CD8＋、CD4＋/CD8＋水平。结果 与模型组比较，正常电针组、假手术电针组乳头高度[（1.22±0.21）mm、（1.24±0.20）mm比（1.53±0.16）mm]显著降低（P＜0.01）；与模型组比较,正常电针组、假手术电针组乳房外观、乳腺组织形态及超微结构明显改善，迷切电针组有一定的改善，迷切不电针组几乎无改善；与模型组比较，正常电针组、假手术电针组、迷切电针组血清中CD3＋含量[（61.5±10.5）%、（61.1±12.4）%、（59.8%±11.8）%比（57.7±9.4）%]、CD4＋含量[（37.2±8.9）%、（37.9±10.9）%、（36.1%±12.2）%比（34.6±6.9）%]、CD4＋/CD8＋[（1.59±0.8）、（1.58±0.8）、（1.38±0.6）比（1.23±0.7）]升高（P＜0.01或P＜0.05）；与模型组比较，正常电针组、假手术电针组、迷切电针组血清中CD8＋含量[（24.2±11.3）%、（24.5±10.6）%、（25.5%±8.8）比（27.7%±9.9）%]降低（P＜0.01或P＜0.05）。结论 “通调针法”电针对MGH大鼠的作用机制与迷走神经有关，可能是通过对迷走神经进一步调节T淋巴细胞CD3＋、CD4＋、CD8＋、CD4＋/CD8＋水平。
Objective The experiment was designed to study the therapeutic mechanism of ＂Tong Tiao＂ acupuncture in the treatment of mammary gland hyperplasia （MGH） on the rat model. Methods A total of 75 healthy unpregnant female SD rats were randomly divided into six groups： blank, model, control group, vagus cutting therapy, sham operation, and vagus cutting with non-treatment group. All the groups except the blank group were prepared by MGH rat model. The vagus cutting therapy and vagus cutting with non-treatment group were treated by cutting the right cervical vagus nerve, while the sham operation group was treated by exposing the vagus nerve without any operation. All the treatment groups were treated with ＂Tong Tiao＂. The rat papillary height was measured. The breast tissue morphology and breast ultrastructure were observated. And the flow cytometry in rat peripheral blood T lymphocyte subsets CD3＋, CD4＋, CD8＋, CD4＋/CD8＋ were calculated. Results Compared with the model group, the papillary height （1.22 mm ± 0.21 mm, 1.24 mm ± 0.20 mm vs. 1.53 mm ± 0.16 mm） in the normal treatment and the sham treatment group decreased significantly the vagus cutting therapy and vagus cutting non-treatment group showed no difference （P〉0.05）. Compared with the model group, the Mammary gland morphology and ultrastructure in the normal treatment group, the sham operation group and the vagus cutting therapy groups were improved, but the vagus cutting non-treatment group showed on improvement. Compared with model group, the serum CD3＋（61.5% ± 10.5%, 61.1% ± 12.4%, 59.8% ± 11.8% vs. 57.7% ± 9.4%）, CD4＋（37.2% ± 8.9%, 37.9% ± 10.9%, 36.1% ± 12.2% vs. 34.6% ± 6.9%）, CD4＋/CD8＋（1.59 ± 0.8, 1.58 ± 0.8, 1.38 ± 0.6 vs. 1.23 ± 0.7） in the normal treatment group, the sham treatment group and vagus cutting therapy group, elevated significantly （P〈0.01, P〈0.05）, while the vagus cutting non-treatment group showed no difference （P〉0.05）. Compared with model group, the serum CD8＋ （24.2% ± 11.3%, 24.5% ± 10.6%, 25.5% ± 8.8% vs. 27.7% ± 9.9%） in the normal treatment group, sham treatment group and vagus cutting therapy group decreased significantly lower （P〈0.01, P〈0.05）, but vagus cutting non treatment group showed no difference （P〉0.05）. Conclusions the mechanism of acupuncture treatment for MGH may be related to the vagus nerve by regulating the CD3＋, CD4＋, CD8＋ and CD4＋/CD8＋ levels of T lymphocytes.
出处
《国际中医中药杂志》
2018年第1期42-47,共6页
International Journal of Traditional Chinese Medicine
基金
基金项目：国家白然科学基金面上项目（81473782）
陕西中医药大学自然科学创新基金项目（14XJZR05）
陕西省科技,Jj自然科学基础研究计划面上项目（2017JM8045）
关键词
电针
迷走神经
乳腺增生
T淋巴细胞亚群
大鼠
Electroacupuncture
Vagus nerve
Mammary gland hyperplasia
T lymphocyte subsets
Rats
