摘要
目的:探讨甲状腺乳头状癌(PTC)组织BRAF蛋白的表达情况及与临床指标的相关性。方法 :选择2015年6月—2016年6月我院诊治的120例PTC患者为观察组,另选择同期诊治的非PCT甲状腺癌患者57例为对照组,采用免疫组化法检测两组肿瘤组织中BRAF蛋白表达,并分析BRAF蛋白阳性表达与PTC临床指标的相关性。结果:观察组患者BRAF蛋白阳性率为74.17%,阴性率为25.83%;对照组患者BRAF蛋白阳性率为7.02%,阴性率为92.98%。两组间比较差异有统计学意义(P<0.01);年龄≤45岁、肿瘤直径>1 cm、TNM分期为Ⅲ~Ⅳ期的PTC患者的BRAF蛋白阳性率分别为96.43%、82.35%、95.65%,明显高于年龄>45岁、肿瘤直径≤1 cm、TNM分期为Ⅰ~Ⅱ期的67.39%、63.46%、69.07%,差异有统计学意义(P<0.05)。结论 :PTC患者肿瘤组织中BRAF蛋白阳性率明显高于非PTC甲状腺癌患者,PTC患者BRAF蛋白阳性表达与年龄、肿瘤直径和肿瘤分期有关。
Objective： To explore the Papillary thyroid carcinoma（PTC） in patients with the ex- pression of BRAF protein and correlation with clinical indicators. Methods： During the selection from June 2015 to June 2016 in our hospital diagnosis and treatment of 120 patients with PTC as the observation group, the other choice during the same period in our hospital make a diagnosis and give treatment of PCT 57 cases as control group, patients with thyroid cancer by immunohistochemical method to detect mutated BRAF protein expression in two groups of patients, and analyze the BRAF protein positive expression and the correlation of PTC clinical indicators. Results： Observation group of patients BRAF protein positive rate was 74.17%, the negative rate was 25.83%, controlgroup patients BRAF protein positive rate was 7.02%, the negative rate was 92.98%, the compari- son between groups have significant statistical difference（P〈0.01）; Age≤45 years, diameter of tu- mor〉l cm, TNM staging for Ⅲ-Ⅳ stage of PTC patients of BRAF protein positive rate were 96.43%, 82.35%, 95.65%, significantly higher than the age〉45 years, tumor diameter〈lcm, TNM staging for Ⅰ-Ⅱ stage were 67.39%, 63.46%, 69.07%, and the corresponding comparison have significant statistical difference（P〈0.05）. Conclusion： PTC mutated BRAF protein positive rate was signifi- cantly higher in patients with non PTC thyroid cancer patients, patients with PTC BRAF protein posi- tive expression related to age, tumor diameter and tumor stage.
出处
《中国现代普通外科进展》
CAS
2017年第10期773-776,共4页
Chinese Journal of Current Advances in General Surgery
关键词
甲状腺乳头状癌
BRAF蛋白
免疫组化法
临床病理因素
Papillary thyroid carcinoma ,BRAF protein ,Immunohistochemical method ,TumordiameteroClinical stage,Lymph node metastasis
