摘要
甲基丙烯酸环氧丙醋(glycidyl methacrylate,GMA)在航空工业、高分子合成工业和人们日常生活中有着广泛的用途,其结构式为:系双功能团单体。本研究组前已报告,在Ames试验中,GMA能诱发鼠伤寒沙门氏菌碱基置换型突变,并能诱发小鼠骨髓多染红细胞微核率升高,从而证明了GMA的诱变作用。在此基础上,拟对其诱变机理作深入探讨。本文采用质粒PBR 322作为分子靶,从分子生物学和遗传毒理学角度探讨GMA对靶分子上抗药性基因表现型的改变及此种改变的可遗传性。
A mutagen, glycidyl methacrylate (GMA), discovered by us a few years ago has been used to investigate the mutation mechanism of drug--resistance genes of plasmid pBR322. The results indicated that GMA binds strongly to pBR322 DNA, and this binding decreased the relative transformation efficiency using E. coli HB1O1 strain on LB plates containing ampicillin (Ap) or tetracy- cline (Tc). The mutants, A^RpT^Sc, A^SpT^Rc and A^SpT^Sc. have been isolated and their drug-resistance proved to be heritable.
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
1991年第4期309-312,共4页
Acta Academiae Medicinae Sinicae
基金
国家自然科学基金
