摘要
目的探讨甲基丙烯酸环氧丙酯（GMA）对人支气管上皮细胞周期及凋亡的影响。方法人支气管上皮细胞（16HBE）经1-16μg/ml剂量的GMA染毒不同次数后,应用流式细胞仪（FCM）检测细胞周期及凋亡率的改变,同时测定细胞分裂指数（MI）。结果经1次染毒处理后,随着染毒剂量的增加,G0/G1期细胞显著减少（P〈0.01）,S期和G2/M期细胞显著增加,凋亡细胞数增多,细胞分裂指数下降;随着染毒次数的增加,各剂量组细胞周期均明显阻滞于G0/G1期,但高剂量组细胞仍表现出S期和G2/M期增多现象;经3次染毒后,高剂量组细胞出现凋亡率下降、分裂指数升高现象。结论经GMA染毒处理后,16HBE细胞周期由G0/G1期向S期和G2/M期移动而呈现增殖性改变。
Objective To investigate the effect of glycidyl methacrylate（GMA） on cell cycle and apoptosis in human bronchial epithelial cells. Methods Flow cytometry was applied to determine cell cycle and apoptosis of 16HBE cells treated at the doses of 1 - 16 μg/ml GMA for different time. Mitotic index （MI） of cell was also analysed. Results When 16HBE cells were treated by GMA for 72hr, cells in G0/G1 phase decreased, but cells in S and G2/M phase increased. The increases of apoptosis rates and the decreases of mitotic index could also be observed. As treatment went on, all groups cells were stopped at G0/G1 phase, but at the dose of 16 μg/ml the cell increases of S and G2/M phase were observed in comparison with the controls. After cells were treated for 3 times, the decreases apoptosis rates and the increases of mitotic index of cell treated by high dosage were observed. Conclusion The 16HBE cells treated by GMA could result in cell cycle transition from G0/G1 phase to S and G2/M phase. It was suggested that the cell proliferation was stimulated. Uncontrolled cellular proliferation may be responsible for malignant transformation induced by GMA.
出处
《卫生研究》
CAS
CSCD
北大核心
2007年第4期404-406,413,共4页
Journal of Hygiene Research
基金
国家自然科学基金资助项目(No.30671789)
