摘要
为探究绞股蓝总皂苷调节自噬小体对动脉粥样硬化的防治作用。本研究分体内、体外实验。体内实验:30只健康Apo E-/-小鼠随机分为3组,模型组、绞股蓝总皂苷组和辛伐他汀组,每组10只。10只C57BL/6J小鼠为正常组。模型制备及给药干预完成后,全自动生化分析仪检测各组小鼠血脂水平,Western blot技术检测各组小鼠主动脉Atg3、Atg4c、Atg5、Atg12蛋白表达情况。体外实验:培养EA.hy926细胞,随机分为正常组、模型组、绞股蓝总皂苷组、人参皂苷GRb3组和绞股蓝皂苷XILX组。Western blot技术检测各组细胞自噬小体形成信号通路相关蛋白Atg3、Atg4c、Atg5、Atg12的表达情况。结果发现,绞股蓝总皂苷能够降低Apo E-/-小鼠血清中的TG、TC、LDL-C水平(P<0.01),提高血清HDL-C水平(P<0.01)。且绞股蓝总皂苷能够提高Apo E-/-小鼠主动脉Atg3、Atg4c、Atg5、Atg12蛋白表达水平(P<0.05或P<0.01)。绞股蓝总皂苷、人参皂苷GRb3和绞股蓝皂苷XILX能够提高ox-LDL诱导的EA.hy926细胞自噬小体相关蛋白Atg3、Atg4c、Atg5、Atg12的表达水平(P<0.01)。且绞股蓝总皂苷效果最好。以上结果说明,股蓝总皂苷可能通过促进自噬小体形成,降低Apo E-/-小鼠血清血脂水平,保护EA.hy926细胞抗内皮损伤,进而发挥其防治AS的生物学作用,且在该过程中人参皂苷GRb3和绞股蓝皂苷XILX可能是绞股蓝总皂苷中发挥关键作用的有效成分。
Abstract ：To investigate the effect of gypenosides on the prevention and treatment of atherosclerosis by autophagosome. This study was divided into in vivo and in vitro experiment. In vivo, thirty healthy Apo E ＂j＂ mice were randomly divided into three groups ,namely model group, gypenosides group and simvastatin group ,each group has ten mice. Ten C57BL/ 6J mice were control group. After the model preparation and the drug intervention was completed, the automatic biochem- ical analyzer was used to test the levels of serum lipids, and western blot was used to detect the expressions of atg3, Atg4c ,Atg5 and Atgl2 protein in the aorta of mice. In vitro, EA. Hy926 cells were cultured and were randomly divided into control group, model group, gypenosides group, ginsenoside GRb3 group and gypenoside XILX group. In addition, the western blot was used to detect the expressions of Atg3 ,Atg4c ,Atg5 and Atgl2 protein of autophagosomes form signaling pathways. The results showed that, gypenosides can reduce the levels of TG, TC and LDL-C （ P 〈 0.01 ）, improve the level of HDL-C （P 〈 0.01 ） in serum of ApoE-/-mice. Furthermore, gypenosides can increase the expression level of Atg3 ,Atg4c ,Atg5 and Atgl2 protein in the aorta of ApoE -/- mice （P 〈 0.05 or P 〈 0.01 ）. Gypenosides, ginsenoside GRb3 and gypenoside XILX can increase the expression levels of Atg3 ,Atg4c,Atg5 and Atgl2 in ox-LDL-induced EA. hy926 cells （P 〈 0.01 ）, in which the effect of gypenosides was the best. The above results showed that, gypenosides may protect EA. Hy926 cells against endothelial injury by promoting the formation of autophagosome, reducing the ser- um lipid level of ApoE -/- mice, and then play its biological role in the prevention and treatment of AS. GinsenosideGRb3 and gypenoside XILX were possibly the active constituents of G. pentaphyllum in this process.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2017年第12期2112-2116,2127,共6页
Natural Product Research and Development
基金
辽宁省科学技术计划(20170540592)
辽宁省教育厅一般项目(L201613)
第60批中国博士后科学基金面上资助项目(2016M601331)
国家自然科学基金青年基金(81300229)
沈阳市科技计划(17-139-8-00)
