摘要
目的：研究罗布麻叶和白麻叶中主要共有次生代谢产物的积累，揭示二者主要差异，并阐明一定的规律。方法：在7，8，9月共3个月份，共采集不同生境的罗布麻和白麻叶片33份，应用超高效液相色谱法同时测定叶片中的绿原酸、芦丁、金丝桃苷、异槲皮素、紫云英苷、槲皮素、山柰酚的含量，用描述统计、相关分析、聚类分析进行数学分析。结果：17个白麻样品和16个罗布麻样品中绿原酸含量标准差分别为13．24，12．59mg·g^-1，二者金丝桃苷、芦丁、异槲皮素、紫云英苷含量的标准差分别为1．00，4．76，3．62，0．23mg·g。和0．27，0．82，0．59，0．03mg·g^-1；白麻和罗布麻待测组分含量分别呈现为则呈现7月低，8月高，9月低和7月至9月依次增加的整体趋势；33份样品在类间距离等于15时，可以分为4类。结论：从主要成分含量的稳定性考虑，开发时首选罗布麻叶；虽然罗布麻叶和白麻叶的主要成分存在一定差异，但因为生境与采收季节的关系，单纯以主要化学成分的含量不能有效区分罗布麻叶与白麻叶，二者在没有严格要求时，尤其是主要关注实验所考察的这些成分的利用时，可以混用：从种植地域考虑，阿勒泰、吉木乃、伊犁、长春是较佳的选择。
Objective： To study on the accumulation of secondary metabolites in leaves of Apocynum ventum and Poacynum hendersonii, reveal the main difference s between them and clarify certain regularity. Method： In July, August and September, 33 samples in different habitats were collected, and then the contents of chlorogenic acid, rutin, hyperoside, isoquercetin, astragulin, quercetin and kaempferol in leaves were detected by ultra performance liquid chromatography （UPLC）. Finally, the data were analyzed by descriptive statistics, correlation analysis and cluster analysis. Result： The standard deviation of chlorogenic acid content in 17 samples of A. ventum and 16 samples of P. hendersonii was 13.24 and 12.59 mg.g-Z respectively, and the standard deviation of rutin, hyperoside, isoquercetin, astragulin, and kaempferol of them was 1.00, 4.76, 3.62, 0.23 mg.g and 0.27, 0.82, 0.59, 0.03 mg·g . The main components content in leaves of Poacynum displayed low （July） -high （August） -low （September） , while Apocynum displayed gradual increase in these three months. 33 samples with distance between clusters equal to 15, can be divided into four categories. Conclusion： Considering from the stability of the main component content, Apocynum leaf was preferred in development. Although the main compositions of Apocynum leaves and Poacynum leaves had certain differences, the content of main chemical composition was not effective to distinguish the Apocynum and Poacynum because of the influence by habitat and the harvest season. In case of no strict requirements, especially when focusing on the main compounds, they can be used alternatively. Altay, Jeminay, Ili and Changchun would be the better planting areas in respect of planting areas.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2018年第2期62-68,共7页
Chinese Journal of Experimental Traditional Medical Formulae
基金
新疆维吾尔自治区科技计划项目(201232123)
关键词
罗布麻
白麻
统计分析
次生代谢
绿原酸
黄酮
Apocynum ventum
Poacynum hendersonii
statistical analysis
secondary metabolites
chlorogenic acid
flavonoid
