摘要
以灰树花为原料,探究灰树花蛋白亚铁螯合肽的最佳制备条件,并对其抗氧化活性进行评价。从6种蛋白酶中筛选出酶解最佳用酶,在此基础上,以亚铁螯合能力为响应值,进行单因素和正交试验,同时研究了灰树花蛋白亚铁螯合肽对1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基、羟自由基的清除效果。结果表明,碱性蛋白酶酶解物的亚铁螯合能力最强,在pH 9、加酶量1 800 U/g、酶解温度55℃、底物质量浓度0.5 g/100 mL、酶解时间2.5 h的条件下,灰树花蛋白亚铁螯合肽的亚铁螯合能力最强,可达1.854 mg/g,此时水解度为6.14%。灰树花蛋白亚铁螯合肽对DPPH自由基和羟自由基有较强的清除效果,且与多肽质量浓度呈正相关。结果显示,灰树花蛋白亚铁螯合肽具有良好的亚铁螯合能力,并具有较好的抗氧化活性,有望发展为新型的膳食补充剂。
In this study,we optimized the conditions for the enzymatic preparation of iron-chelating peptides from the fruiting bodies of Grifola frondosa and evaluated their antioxidant capacities.Six commercial proteases were screened for their suitability to hydrolyze Grifola frondosa protein,which was evaluated according to iron-chelating activity.Onefactor-at-a-time and orthogonal array designs were employed to optimize the hydrolysis conditions based on iron-chelating activity.Meanwhile,the antioxidant capacity was studied using 1,1-diphenyl-2-picrylhydrazyl（DPPH） and hydroxyl radical scavenging assays.The results showed that peptides with the highest iron-chelating activity were obtained with alkaline protease.The optimal hydrolysis conditions were determined as follows： pH value,9; alkaline protease dose,1 800 U/g; temperature,55 ℃; substrate concentration,0.5 g/100 m L; and hydrolysis time,2.5 h.Under these conditions,the ironchelating activity and the degree of hydrolysis were 1.854 mg/g and 6.14%,respectively.The prepared peptides showed scavenging activity against DPPH and hydroxyl radicals in a concentration-dependent manner.In addition,the peptides possessed good iron-chelating ability and antioxidant activities,and could thus hold promise as a novel dietary supplement.
作者
赵聪
程晨
尹诗语
李云
朱灿
黄晓伶
陈贵堂
ZHAO Cong;CHENG Chen;YIN Shiyu;LI Yun;ZHU Can;HUANG Xiaoling;CHEN Guitang(School of Engineering, China Pharmaceutical University, Nanjing 211198, China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2018年第2期73-79,共7页
Food Science
基金
国家自然科学基金青年科学基金项目(31201344)
中国药科大学中央高校基本科研业务费重点项目(2016ZZD001)
中国药科大学药学基地科研训练及科研能力提高项目(J1310032)
关键词
灰树花
酶解
多肽
亚铁螯合能力
抗氧化
Grifola frondosa
hydrolisis
peptides
iron-chelating activity
antioxidant activity
