摘要
为获得禽腺病毒毒株,将临床疑似Ⅰ群禽腺病毒感染病例的肝组织,通过接种SPF鸡胚进行病毒分离,用PCR方法对分离产物进行扩增,将PCR产物进行序列测定,对测定的序列在NCBI网上进行分析,结果显示,从病料可分离出病毒,用PCR方法特异性地扩增出了与设计片段大小相一致的片段,序列分析表明,分离株序列与禽腺病毒SD1-15分离株序列同源性99%,说明分离株为Ⅰ群禽腺病毒血清4型。
In order to obtain the virus of fowl adenovirus,liver tissue collected from chicken with clinical suspected fowl adenovi- rus infection, we isolate virus throughinoculate collected specimens to SPF chicken embryo, and then identify the collected specimens and chicken embryo allantoic fluid using PCR. The PCR product was sequenced. The results showed that the allantoie fluid was PCR positive for duck flavivirus. The sequence was 99% identity between tested virus and fowl adenovirus 4 isolate SD1 - 15 hexon pro_ tein gene. This indicated that the isolated virus was fowl adenovirus.
出处
《中国兽医杂志》
CAS
北大核心
2017年第10期45-47,I0003,共4页
Chinese Journal of Veterinary Medicine
基金
国家蛋鸡产业技术体系合肥综合试验站项目(CARS-41-S13)
关键词
禽腺病毒
分离
鉴定
fowl adenovirus
isolation
identification
