摘要
目的通过研究维生素D受体（VDR）基因多态性在长沙地区慢性阻塞性肺疾病（COPD）患者中的分布，探讨COPD发病的遗传易感因素。方法应用聚合酶链反应-限制性长度多态性的方法检测120例COPD患者及120例正常对照成人的维生素D受体Apa I、Bsm I基因多态性，比较两组的基因型及其等位基因的分布频率。结果两组Apa，基因多态性及其等位基因的分布频率差异均有统计学意义（P〈0．05），而Bsm I基因分布频率差异无统计学意义（P〉0．05）。结论VDR-Apa，基因可能是COPD的遗传易感基因，而VDR．Bsm，基因多态性可能与COPD无关。
Objective To investigate the genetic predisposing factor of chronic obstructive pulmo- nary disease （COPD） by studying the distribution of vitamin D receptor （VDR） gene polymorphisms in patients with COPD in Changsha. Methods Totally 120 COPD patients and 120 volunteers were enrolled in the study. Polymorphisms of VDR genes including Apa I and Bsm I were detected with polymerase chain reaction-restriction fragments length polymorphism （RFLP）. Results There existed statistical difference in polymorphism of Apa I gene and frequency of distribution on its allele between two groups （P 〈 0. 05 ）, while there is no significant difference on Bsm I gene between the two groups （ P 〉 0. 05 ）. Conclusions COPD is associated with the polymorphism of VDR-Apa I. There may be no relationship on VDR-Bsm I between two groups.
出处
《中国医师杂志》
CAS
2017年第11期1615-1617,共3页
Journal of Chinese Physician
基金
湖南省卫生计生委科研计划课题项目（B2016165）
