摘要
利用高效液相色谱联合二极管阵列技术(HPLC-DAD)建立一种可以同时检测不同苹果醋和甘蔗醋中11种酚类物质的分析方法。使用OMNI Hubble C_(18)色谱柱(250 mm×4.6 mm,5μm),流动相A为甲醇-甲酸(100∶2,v/v),B为10 mmol/L磷酸氢二钾-磷酸水溶液(200∶1,v/v),流速为1.0 m L/min,进样量为20μL,梯度洗脱,柱温为35℃,二极管阵列扫描波长范围210~400 nm。应用本方法可以检测出不同苹果醋和甘蔗果醋中香草醛、香豆素、绿原酸、咖啡酸、阿魏酸、对香豆酸、芹菜素、木犀草素、肉桂酸、山奈酚、芒柄花黄素等11种酚类物质,其中甘蔗醋中富含绿原酸等10种酚类物质成分。本方法回收率在84.1%~118.2%之间,结果准确可靠,简单易行,适合用于各类果醋中酚类物质的分析检测。
A new method for simultaneous determination of 11 phenolic compounds in fruit vinegar,such as different kinds of apple vinegar and sugarcane vinegar, by high performance liquid chromatography- diode array detector （ HPLC - DAD ） was established in this study.The phenolic compounds were separated by OMNI BOND with Hubble C18 column（250 mm×4.6 mm, 5 μm）at 35 ℃ .The mobile phase A was MeOH-HCOOH solution （100： 2, v/v）and the mobile phase B was 10 mmol/L K2 HPO4 -H3 PO4 （200：1 , v/v） , eluted at a flow rate of 1.0 mL/min by a gradient elution program.The injection volume was 20 μL and the eluted components were monitored at 210-400 nm.Under the optimized condition, 11 phenolic compounds in different kinds of apple vinegar and sugarcane vinegar were separated and simultaneous determined, including coumarin, vanillin,chlorogenic acid, caffeic acid, ferulic acid, p- coumaric acid, apigenin, luteolin, cinnamic acid, kaempferol and formononetin,in which 10 phenolic components were found and determined in sugarcane vinegar. The recovery of the above method was 84.1%- 118.2%. The method is accurate, reliable, simple, feasible and suitable for determination of phenolic compounds in fruit vinegar.
出处
《食品工业科技》
CAS
CSCD
北大核心
2017年第23期210-213,220,共5页
Science and Technology of Food Industry
基金
国家星火计划项目(2015GA790013)
广西科技计划项目(桂科AB16380244、桂科合14123001-10)
南宁市西乡塘区科技计划项目(201720103)
广西农业科学院基本科研业务专项(桂农科2015YZ02)
广西农业科学院科技发展基金项目(桂农科2017JM60)
关键词
苹果醋
甘蔗醋
酚类
高效液相色谱
二极管阵列
apple vinegar
sugarcane vinegar
phenolic compounds
high performance liquid chromatography
diode arraydetector （DAD）
