摘要
目的:优化复合酶解法辅助提取云芝多糖的提取工艺,并研究其抗氧化活性。方法:在单因素实验基础上,利用Box-Behnken方法进行酶浓度、p H、酶解时间和酶解温度四因素三水平实验设计,以多糖得率为响应值,采用响应面分析优化云芝多糖的提取条件。采用对云芝多糖清除DPPH自由基能力、清除羟自由基(·OH)能力和清除超氧阴离子(O_2^-·)能力的测定评价云芝多糖的抗氧化活性。结果:最佳提取条件为p H5.50,酶解时间37 min,酶解温度52℃,酶浓度2.50%,理论上云芝多糖的提取率为9.87%,验证实验的实际提取率为9.58%,与传统的热水浸提法提取率相比较,提高了43.63%。云芝多糖清除·OH、O_2^-·和DPPH自由基的IC_(50)分别为0.80、0.75、0.75 mg/m L,抗氧化活性研究中云芝多糖的各抗氧化活性均随多糖浓度的增加而上升。结论:复合酶解法辅助提取条件温和,方法简单,效率高,可在云芝多糖实际提取工艺中得到应用,得到的云芝多糖具有良好的抗氧化能力,可进一步的开发利用。
Objective： To optimize the extraction process of Coriolus versicolor polysaccharides by compound enzymatic hydrolysis, and study its antioxidant activity. Methods ： On the basis of single factor experiment, a text of four factors at three different levels which used the method of Box- Behnken was designed, including the enzyme concentration, pH, time and temperature of enzymatic hydrolysis, meanwhile the respond value was polysaceharide rate, with the response surface methodology to optimize the extraction conditions of Coriolus versicolor polysaecharide.By the research of the scavenging DPPH free radicals, hydroxy radica,we can discuss the antioxidant ability of Coriolus versicolor polysaceharides.Results ：The optimum extraction conditions are obtained： at the pH5.50 and 52℃, the enzymatic hydrolysis time was 37 min, and the enzyme concentration was 2.50% ,the extraction rate could reach 9.87% in theory, but the actual extraction rate was 9.58% ,which raised 43.63% compared with the traditional hot water extraction.The half maximal inhibitory concentration of OH, 02- and DPPH radicals were 0.80, 0.75, 0.75 mg/mL, respectively. The total antioxidant activity increased with the increasing concentration of polysaecharides. Conclusion： The method of compound enzymatic hydrolysis has the advantages of mild conditions,simple reaction and high efficiency, which can apply in the practical extraction process of Coriolus versicolor polysaeeharide.It has good antioxidant activity and is worth to develop for application.
出处
《食品工业科技》
CAS
CSCD
北大核心
2017年第23期176-181,共6页
Science and Technology of Food Industry
基金
大连民族大学大学生创新创业训练计划项目(XA201603105
201712026099)
财政专项-中央高校基本科研业务费项目(DC201501020101
DC201501020301
关键词
云芝多糖
酶解法
提取条件
响应面法
抗氧化活性
Coriolus versicolor polysaccharide
enzymatic hydrolysis
extraction conditions
response surface method
antioxidantactivity
