摘要
目的探讨程序性死亡分子1(PD1)在类风湿关节炎(RA)患者外周血CD4^+T和CD8^+T细胞上的表达及临床意义。方法应用流式细胞仪检测66例RA患者和30例健康对照者外周血CD4^+T和CD8^+T细胞上PD1表达(平均荧光强度和百分率),比较RA组和健康对照组之间CD4^+T和CD8^+T细胞上PD1表达水平,分析其与RA实验室检查指标的相关性。结果 (1)RA患者CD4^+T、CD8^+T细胞PD1表达的平均荧光强度(MFI)显著高于对照组,差异均有统计学意义(P<0.05);RA患者CD4^+PD1^+T细胞百分率显著高于对照组,差异有统计学意义(P<0.05)。(2)RA患者外周血CD4^+T、CD8^+T细胞PD1表达的MFI与DAS28评分呈正相关性(r=0.49,P<0.000 1;r_s=0.40,P=0.0008)。(3)RA患者外周血CD4^+T细胞PD1表达的MFI与血沉(ESR)、C-反应蛋白(CRP)呈正相关性(r=0.45,P<0.000 1;r=0.39,P=0.001);RA患者外周血CD8^+T细胞PD1表达的MFI与CRP呈正相关性(r_s=0.40,P=0.0009);RA患者外周血CD4^+PD1^+T细胞百分率与CRP呈正相关性(r=0.24,P=0.048)。(4)RA患者外周血CD8^+T细胞PD1表达的MFI与类风湿因子(RF)呈正相关性(r_s=0.32,P=0.009);RA患者外周血CD4^+PD1^+T细胞百分率与RF呈正相关性(r=0.27,P=0.030)。结论 RA患者外周血CD4^+T和CD8^+T细胞PD1表达异常,与疾病的活动性及抗体产生有关。
Objective To investigate the expression of PD1 on CD4^＋T and CD8^＋T from the patients with rheumatoid arthritis（ RA） and to analyze the clinical relevance to disease severity. Methods The expression of PD1 on CD4^＋T and CD8^＋T were examined from 66 RA patients and 30 healthy controls（ HC） by the technique of flow cytometry. The expression of PD1 including mean fluorescence intensity（ MFI） on CD4^＋T and CD8^＋T and percentage of CD4^＋PD1^＋T and CD8^＋PD1^＋T were compared between RA patients and HC. Moreover,its correlation with laboratory inspection was analyzed. Results（1） The MFI of PD1 on CD4^＋T and CD8^＋T from RA patients significantly elevated compared with HC（ P〈0. 05）,and the percentage of CD4^＋PD1^＋T from RA patients significantly elevated compared with HC（ P〈0. 05）.（2） The MFI of PD1 on CD4^＋T and CD8^＋T in RA patients were positively associated with DAS28（ r = 0. 49,P〈0. 000 1;r_s= 0. 40,P = 0. 000 8）.（3） The MFI of PD1 on CD4^＋T in RA patients were positively associated with CRP and ESR（ r = 0. 45,P〈0. 000 1;r = 0. 39,P = 0. 001）. The MFI of PD1 on CD8^＋T in RA patients was positively associated with CRP（ r_s= 0. 40,P = 0. 000 9）. The percentage of CD4^＋PD1^＋T in RA patients was positively associated with CRP（ r = 0. 24,P = 0. 048）.（4） The MFI of PD1 on CD4^＋T in RA patients were positively associated with RF（ r_s= 0. 32,P = 0. 009）. The percentage of CD4^＋PD1^＋T in RA patients was positively associated with RF（ r = 0. 27,P = 0. 030）. Conclusion The aberrations expression of PD1 on CD4^＋T and CD8^＋T are observed in patients with RA. Increased expression of PD1 on CD4^＋T and CD8^＋T are correlated with disease activity,and also the production of antibody.
出处
《安徽医科大学学报》
CAS
北大核心
2017年第12期1854-1859,共6页
Acta Universitatis Medicinalis Anhui
基金
国家自然科学基金(编号:81360459)
江西省青年科学基金(编号:20151BAB215031)
