摘要
目的研究血管生成相关因子血管内皮生长因子(vascular endothelial growth factor,VEGF)、细胞间黏附分子-1(intercellular adhesion molecule-1,ICAM-1)、血管细胞黏附分子-1(vascular cell adhesion molecule-1,VCAM-1)在口腔扁平苔藓患者血清和组织中的表达情况。方法 30例扁平苔藓患者纳入扁平苔藓组,15例健康志愿者作为对照(正常组)用于血清收集,收集扁平苔藓组织患者活检组织30例及外科手术切除的多余正常口腔黏膜组织15例,应用免疫组织化学技术分析VEGF、ICAM-1、VCAM-1分别在扁平苔藓病损局部组织及正常黏膜组织中的表达情况。结果 ELISA结果显示VEGF在2组血清中表达差异无统计学意义(t=1.837,P=0.074),而ICAM-1(t=2.730,P=0.009)、VCAM-1(t=2.167,P=0.035)在扁平苔藓组血清中的表达明显高于正常组。免疫组织化学染色结果显示VEGF、ICAM-1、VCAM-1在口腔黏膜正常上皮中呈现少量阳性表达或阴性表达,而在扁平苔藓组织中VEGF(χ~2=33.632,P<0.05)、ICAM-1(χ~2=45.000,P<0.05)、VCAM-1(χ~2=37.286,P<0.05)表达较正常黏膜组织均出现上调趋势。结论在扁平苔藓患者的血清和病变组织中,血管生成相关因子表达上调。
Objective To detect the expression of vascular endothelial growth factor（VEGF）, intercellular adhesion molecule-1（ICAM-1）, vascular cell adhesion molecule-1（VCAM-1） in oral lichen planus（OLP）. Methods Thirty OLP patients and fifteen healthy subjects were enrolled in the study. Serum were collected from 15 healthy volunteers as controls. Normal tissues were collected from surgical department as immunohistochemical analysis. The levels of VEGF,ICAM-1, VCAM-1 in serum were measured by ELISA. Immunohistochemical analysis of VEGF, ICAM-1, VCAM-1 were carried out by the means of primary antibodies and anti-VEGF, anti-CD106 antigen（VCAM-1） and anti-CD54 antigen（ICAM-1）. Results ELISA results showed no expression differences for VEGF between the two groups. Whereas,the levels of ICAM-1, VCAM-1 in OLP group were significantly higher than those in control group（P 0.05）. Immunohistochemical results reveal the presence of a significant angiogenesis in OLP patients through the immunoexpression of VEGF, ICAM-1, VCAM-1 according to the percentage of stained cells（P 0.05）. Conclusion Regarding the results,it seems that high expression of VCAM-1 and ICAM-1 are related to oral lichen planus.
出处
《口腔疾病防治》
2017年第11期712-717,共6页
Journal of Prevention and Treatment for Stomatological Diseases
基金
国家自然科学基金(81500850)
广东省医学科学技术研究基金(A2015537)
