摘要
目的:探讨HBV感染产妇血清和初乳中HBV-DNA载量的相关性。方法:用荧光定量聚合酶链式反应(FQ-PCR)技术对240例HBV感染产妇的血清和初乳进行检测,按产妇血清HBV-DNA载量分为HBV-DNA<5.00E+02IU/mL、5.00E+02~9.99E+03IU/mL、1.00E+04~9.99E+05IU/mL、1.00E+06~9.99E+07IU/mL、≥1.00E+08IU/mL,分析比较产妇血清、初乳中病毒HBVDNA载量。结果:240例HBsAg(+)产妇血清HBV-DNA阳性率为72.9%(175/240),初乳阳性率为30.8%(74/240),两者比较差异有统计学意义(χ~2=112.203,P<0.001);HBsAg(+)产妇血清HBVDNA载量<5.00E+02IU/mL时,其对应的初乳中均未检出HBV-DNA,血清HBV-DNA载量在5.00E+02~9.99E+03IU/mL、1.00E+04~9.99E+05IU/mL、1.00E+06~9.99E+07IU/mL、≥1.00E+08IU/mL区间时,其对应初乳中HBV-DNA检出率分别为4.7%(2/42)、41.8%(23/55)、52.5%(32/61)、100%(17/17),初乳中HBV-DNA≥5.00E+02IU/mL者所占人数比值在血清HBV-DNA不同层级载量组别中比较,差异有统计学意义(χ~2=96.986,P<0.001);当血清载量HBV-DNA≥5.00E+02IU/mL时,初乳中HBV-DNA载量和检出率随血清HBV-DNA载量的升高而增加,二者高度相关(r=0.876,P<0.001)。结论:产妇血清HBV-DNA载量决定其初乳HBV-DNA水平,初乳中HBV-DNA载量低于血清;当产妇血清HBV-DNA载量<5.00E+02IU/mL时,其对应的初乳中均未检出HBVDNA,提示此时母乳喂养发生母婴传播的风险较小。
Objective:To investigate the correlation between HBV-DNA load in serum and colostrum of HBV-infected women and to offer medical workers reference of efficiently nursing and taking immunological methods on these special maternal and their newborns.Methods:Using FQ-PCR to test the serum and colostrum in 240 HBV-infected maternal where they are analyzed in the level of HBV-DNA<5.00 E+02 IU/mL,5.00 E+02~9.99 E+03 IU/mL,1.00 E+04~9.99 E+05 IU/mL,1.00 E+06~9.99 E+07 IU/mL and ≥1.00 E+08 IU/mL.Results:72.9%(175/240)of the 240 samples were HBV-DNA positive in serum while 30.8%(74/240)positive in colostrum,which showed significant difference statistically(χ~2=112.203,P<0.001).HBV-DNA appeared to be negative in colostrum when HBsAg(+)maternal had a HBV-DNA carrying capacity of<5.00 E+02 IU/mL.And with the carrying capacity of HBV-DNA in serum rose up to 5.00 E+02~9.99 E+03 IU/mL,1.00 E+04~9.99 E+05 IU/mL,1.00 E+06~9.99 E+07 IU/mL,≥1.00 E+08 IU/mL,the proportion of HBV-DNA(+)in colostrum come to 4.7%(2/42),41.8%(23/55),52.5%(32/61),100%(17/17)respectively.Also,the proportion of HBV-DNA ≥5.00 E+02 IU/mL were statistically different(χ~2=96.986,P <0.001)when compared with other groups that were divided by the level of HBV-DNA in serum.When the carrying capacity of HBVDNA in serum reached≥5.00 E+02 IU/mL,with highly correlation(r=0.876,P<0.001),it rose along in colostrum.Conclusion:The carrying capacity of HBV-DNA in colostrum of the maternal depends on the capacity in their serum.When HBsAg(+)maternal has a HBV-DNA carrying capacity of<5.00 E+02 IU/mL,it implicates a lower risk to spread HBV through breastfeeding with the evidence that HBV-DNA appears to be negative in colostrum.But the carrying capacity of HBV-DNA in colostrum rises with it in the serum of the maternal,which indicates that there are potential ability to spread through breastfeeding.
出处
《长治医学院学报》
2017年第5期339-343,共5页
Journal of Changzhi Medical College
基金
山西省卫生计生科研课题(2015162)
