摘要
目的观察十溴联苯醚(BDE209)低水平母源性暴露对子一代小鼠学习记忆能力及甲状腺激素分泌的影响。方法无特定病原体级4周龄雌性昆明小鼠自确定交配成功后,随机分为对照组和低、中、高染毒组,每组16只;对照组母鼠灌胃0.01 L/kg体质量花生油,染毒组母鼠根据50、100和300μg/kg体质量灌胃染毒BDE209,1次/d,连续染毒直至子鼠出生21 d,建立妊娠期至哺乳期的暴露模型。染毒结束后,随机选取每组子鼠10只,雌雄各半,观察其体质量及发育状况;采用水迷宫实验检测子鼠的学习记忆能力;采用化学发光免疫分析法测定子鼠血清总三碘甲腺原氨酸(TT3)、游离三碘甲腺原氨酸(FT3)、总四碘甲腺原氨酸(TT4)和游离四碘甲腺原氨酸(FT4)水平;采用比色法测定子鼠海马组织中谷胱甘肽转移酶(GST)和超氧化物歧化酶(SOD)活力、丙二醛(MDA)水平。结果中染毒组子鼠逃避潜伏期长于对照组(P<0.05);高染毒组子鼠逃避潜伏期分别长于对照组和低、中染毒组(P<0.05);高染毒组子鼠平台象限活动时间和穿越平台次数分别少于对照组和低、中染毒组(P<0.05)。随着染毒剂量的增加,子鼠血清中TT3、FT3、TT4和FT4水平逐渐增高(P<0.05),海马组织中GST和SOD活力逐渐降低(P<0.05),MDA水平逐渐增高(P<0.05)。结论低水平母源性BDE209可导致子鼠学习记忆能力下降,并可导致子鼠血清甲状腺激素水平呈剂量依赖性升高,海马组织出现剂量依赖性的氧化应激损伤。
Objective To observe the effects of maternal low-level decabromodiphenyl（ BDE209） exposure on nervous system and secretion of thyroid hormones in offspring mice. Methods Sixty-four specific pathogen free female,aged 4 weeks Kunming mice were used. These mice were randomly divided into control group and low,medium and high exposure groups after successful mating was confirmed. The rats of control group were fed with 0. 01 L/kg body mass of peanut oil.The maternal mice in the experimental groups were given BDE209 at doses of 50,100 and 300 μg/kg body mass by oral gavage once per day. Continuous exposure was given until 21 days after birth of offspring,the exposure model from gestation to lactation was established. At the end of the exposure,10 mice of each group including half female and half male were randomly selected and the body mass and growth development status were observed. Morris water maze was used to examine the learning and memory ability in offspring mice. The serum levels of total triiodothyronine（ TT3）,free triiodothyronine（ FT3）, total tetraiodothyronine（ TT4） and free tetraiodothyronine（ FT4） were measured by chemiluminescence immunoassay. The levels of glutathione transferase（ GST）, superoxide dismutase（ SOD） and malondialdehyde（ MDA） level in hippocampus were measured by colorimetry. Results The escape latency of the medium exposure group was longer than that of the control group（ P〈0. 05）. The escape latency of high exposure group was longer than that of control group,low exposure group and medium exposure group（ P〈0. 05）. The time of quadrant movement and number of crossing the platform in offspring rats in high exposure group were less than that of the control group and the low and medium exposure groups（ P〈0. 05）. The serum levels of TT3,FT3,TT4 and FT4 increased,the activities of GST and SOD in hippocampus tissue decreased,the MDA level increased with the increasing exposure dose（ P〈0. 05）.Conclusion Maternal low-level BDE209 exposure can result in decrease the learning and memory ability of offspring mice. It also can increase the serum thyroid hormone level and induce oxidative stress injury in hippocampus in a dose dependent manner in offspring mice.
出处
《中国职业医学》
CAS
北大核心
2017年第5期547-551,共5页
China Occupational Medicine
基金
国家自然科学基金(81560530)
2016年广西自治区大学生科技创新项目(201610601085)
关键词
十溴联苯醚
学习记忆
甲状腺激素
氧化损伤
Decabromodiphenyl
Learning, memory
Thyroid hormones
Oxidative injury
