摘要
目的研究人乳头瘤病毒31型(HPV31)次要外壳蛋白L2保守中和表位的免疫活性及诱发抗体的中和范围。方法合成法获得HPV31 L2 aa.17-40多肽,用EDC法偶联KLH,联合弗氏佐剂免疫新西兰大白兔,用假病毒中和实验检测免疫血清对来自α4、α7、α9、α10及β1亚属的多个HPV型别的中和抗体。结果 HPV31 L2-KLH偶联肽可在新西兰大白兔体内诱发针对至少17种HPV型别的广谱中和抗体,其中HPV31的中和抗体滴度最高,HPV5/45/57的次之。结论首次发现HPV31 L2保守中和表位免疫血清具有广谱中和活性,为基于该表位的广谱HPV疫苗研发奠定了基础。
Objective To detect the immunogenicity of conservative neutralizing epitope from human papillomavirus type 31（HPV31） minor capsid protein L2,and analyze the neutralizing antibody spectrum of its immune sera.Methods Synthesize HPV31 L2 aa.17-40 peptide and conjugate with KLH by EDC.Immunize New Zealand white rabbits with HPV31 L2-KLH and Freud＇s Adjuvant.Analyze the neutralizing antibody titers of immune sera against HPVs from α4,α7,α9,α10 and β1 species by pseudovirus neutralization assay.Results In rabbits,HPV31 L2-KLH induced broad-spectrum neutralizing antibodies against at least 17 types of HPV,among which the neutralizing antibody titers against HPV31 are the highest,followed by that of HPV5/45/57.Conclusions The broad-spectrum neutralizing activity of the immune sera of HPV31 L2 is stimulated by conservative neutralizing epitope.The results lay the foundation of the development of broad-spectrum HPV vaccines based on HPV31 L2 neutralizing epitope.
出处
《基础医学与临床》
CSCD
2017年第11期1552-1556,共5页
Basic and Clinical Medicine
基金
国家国际科技合作项目(2013DFA32430)
中国医学科学院医学与健康科技创新工程(2016-I2M-3-026)
关键词
人乳头瘤病毒31型
L2保守中和表位
广谱中和抗体
human papillomavirus type 31
L2 conservative neutralizing epitope
broad-spectrum neutralizing antibody
