摘要
对14株分离自四川不同地区的基因C型鸭甲肝病毒(DHAV-C)VP1基因进行了PCR扩增、测序、序列比对分析以及抗原表位分析。结果显示,14株DHAV-C VP1基因核苷酸序列的同源性为92.2%～100%,推导氨基酸序列的同源性为85.8%～100%,与GenBank中的24株DHAV-C VP1基因的核苷酸和氨基酸序列同源性分别为87.9%～99.3%和80.8%～100%。聚类分析显示,四川华阳地区分离的6株DHAV-C的VP1基因与黑龙江分离株Du/CH/LJS/090905同属一支,3株四川邛崃地区分离株与北京分离株C-GY及福建分离株FJ01同属一支,4株四川郫县分离株与1株四川邛崃分离株处于独立的小分支,它们与其他毒株之间存在一定的遗传距离。DHAV-C VP1蛋白由240个氨基酸组成,是由α螺旋、β折叠和无规则卷曲共同组成的混合型蛋白,VP1蛋白氨基酸序列的亲水性、抗原指数和表面可及性均较高,其中212～222位氨基酸区域的亲水性、抗原指数和表面可及性最高,可能是DHAV-C VP1蛋白上的B细胞表位优势区域,为DHAV-C表位疫苗的研究提供了理论依据。结果表明,四川分离株可能由于引种等原因从全国不同地区进入四川境内。
Genetic heterogeneity of genotype C duck hepatitis A virus（DHAV-C） was investigated by direct amplification of VP1 gene of 14 DHAV-C isolates,which were obtained from different districts of Sichuan Province.The 14 DHAV-C isolates showed intragenotypic nucleotide and amino acid divergence with each other and with other strains in the GenBank.The nucleotide sequence homologies among the 14 DHAV-C isolates were 92.2%-100%,and amino acid sequence homologies were 85.8%-100%.The nucleotide sequence homologies among the 14 DHAV-C isolates and 24 DHAV-C strains in the GenBank were 87.9%-99.3%,and amino acid sequence homologies were 80.8%-100%.Cluster analysis showed that 6 DHAV-C isolates from Huayang district in Sichuan Province clustered together with the strain Du/CH/LJS/090905 from Heilongjiang Province,and three DHAV-C isolates from Qionglai districtclustered together with the strains C-GY from Beijing and FJ01 from Fujian Province.One isolate from Qionglai district and 4 from Pixian district in Sichuan Province clustered forming a separate branch.The DHAV-C protein was composed of 240 amino acids,included α helix,β overlaps and random coils.The hydrophilicity,antigen index and surface probability of DHAV-C VP1 protein were all high,and the highest position of that was 212-222 amino acid,which provided a theory basis for epitope vaccine research. This study indicated that the 14 DHAV-C isolated may come from different areas of China by importing breeds.
出处
《中国兽医科学》
CAS
CSCD
北大核心
2012年第3期243-252,共10页
Chinese Veterinary Science
基金
西南民族大学中央高校专项资金项目(11ZYXS22)
西南民族大学中央高校基本科研业务费专项(82000466)
