摘要
目的建立一种研究肠上皮细胞的体外模型—小肠类器官培养体系,探索其相关病理检测技术方法,为肠道相关疾病的体外研究提供便利平台。方法将小鼠肠上皮隐窝分离并培养成小肠类器官,体外模拟肠上皮的生长发育过程。通过制作石蜡切片,探索应用免疫组化技术以及基于基质胶中类器官三维水平免疫荧光技术对相应的增殖与分化信号进行检测。结果探索并建立了小肠类器官体外培养体系,应用石蜡切片免疫组化技术与三维水平免疫荧光技术能够准确检测小肠上皮结构的生长发育状态。结论小肠类器官体外培养体系的建立与免疫检测技术的应用,将逐渐使其成为人们研究肠道相关疾病最为有利的技术手段。
Objective To establish a small intestinal organoid culture system as an in vitro study model of intestinal epithelial cells,and to explore the relevant pathological detection techniques and provide a convenient platform for in vitro study of various intestinal diseases. Methods The mouse intestinal epithelium was isolated and cultured into organoids to simulate the growth and development of intestinal epithelium in vitro. The proliferation and differentiation signals were detected by immunohistochemistry and three-dimensional immunofluorescence technique. Results The culture system of the mouse small intestine epithelium was established. Immunohistochemical staining and three-dimensional immunofluorescence technique were successfully used to detect the growth and development of small intestinal organoids. Conclusions The successfully established mouse small intestinal organoid culture system and application of immunoassay technology will gradually become a most favorable technical means for studies of various intestinal diseases.
出处
《中国实验动物学报》
CSCD
北大核心
2017年第5期513-518,共6页
Acta Laboratorium Animalis Scientia Sinica
基金
国家自然科学基金项目(No.31371455 2014-2017)
关键词
小肠类器官
石蜡切片
免疫组化
免疫荧光
小鼠
Small intestinal organoids
Pathology
Immunohistochemistry
Immunofluorescence
Mouse
