摘要
目的建立急性痛风性关节炎(acute gouty arthritis,AGA)大鼠模型并观察其维持时间。方法采用25 mg/m L尿酸钠(monosodium urate,MSU)晶体混悬液踝关节腔注射复制大鼠AGA模型,多个时间点动态观察8 d,以大鼠受试踝关节局部皮温、肿胀度、步态、关节液炎性细胞及其滑膜组织病理形态学改变等指标判断是否成模及其维持时间。结果造模后3 h,生理盐水组和模型组均可见踝关节肿胀,皮温升高,步态异常,关节液炎性细胞数增多,滑膜组织增生、毛细血管充血、滑膜细胞排列紊乱等炎症表现,两组以上指标与空白组比较差异均有显著性(P<0.01);造模后4 h,生理盐水组以上炎症表现明显减轻,较3 h时差异有显著性(P<0.01),而模型组较3h时加重(P<0.01),并且与生理盐水组比较差异有显著性(P<0.01);造模后24 h,生理盐水组各项指标恢复正常,而模型组炎症继续加重;造模后48~72 h,模型组肿胀、皮温、步态异常等局部炎症达到高峰;造模后96~168h,模型组踝关节局部炎症逐渐减轻,但各项指标与空白组比较差异仍有显著性(P<0.01);造模后192 h,模型组肿胀、皮温、步态异常等外在炎症表现恢复正常,而炎性细胞数及滑膜病理变化与空白组比较差异仍均有显著性(P<0.01)。结论采用MSU晶体混悬液踝关节腔注射可在造模后4 h成功制备并鉴定出AGA大鼠模型,且至少能维持到造模后168 h。
Objective To establish a model of acute gouty arthritis（ AGA） in rats and observe its maintenance time. Methods The AGA model of rats was established by injecting monosodium urate（ MSU） at the concentration of 25mg/mL into the ankle joint cavity. The rats were observed for 8 d at different time points. Skin temperature,degree of joint swelling,gait,inflammatory cells in synovial fluid,histopathological changes of synovial tissue and other indicators were observed to determine whether the modeling and maintenance time were successful. Results At 3 h after modeling,differences in the swelling of ankle joint,increase of skin temperature,abnormal gait,the number of inflammatory cells in synovial fluid,synovial hyperplasia,capillary congestion,and disarrangement of synovial cells in the rats were observed in the saline group and the model group（ P〈0. 01）. At 4 hours after modeling,the above mentioned inflammatory changes in the saline group were significantly reduced,compared with that at 3 h,showing a significant difference（ P〈0. 01）,whilethe inflammatory changes of the model group were increased significantly compared with that at 3 hours（ P〈0. 01）,and showed significant difference compared with the saline group（ P〈0. 01）. At 24 h after modeling,the indexes in the rats of saline group returned to normal,but the inflammation of the model group was increased. At 48-72 h after modeling,the local inflammation such as ankle swelling,skin temperature,and abnormal gait of the rats in the model group reached a peak. The inflammation of the ankle joint in the model group was gradually reduced from 96 to 168 h after the model was established,but there were still significant differences in the indexes compared with the blank group（ P〈0. 01）. At 192 h after modeling,the joint swelling,skin temperature and abnormal gait of the rats in the model group returned to normal,however,there were significant differences in the number of inflammatory cells and the pathological changes of synovial membrane compared with the blank group（ P〈0. 01）. Conclusions A rat model of AGA can be successfully prepared and identified at 4 h after modeling by injection of MSU crystal suspension into the ankle joint cavity. This rat model of AGA can be maintained at least 168 hours after modeling.
出处
《中国实验动物学报》
CSCD
北大核心
2017年第5期494-499,共6页
Acta Laboratorium Animalis Scientia Sinica
基金
国家自然科学基金资助项目(No.81473495)
关键词
急性痛风性关节炎
大鼠模型
尿酸钠
维持时间
Acute gouty arthritis
Rat models
Monosodium urate, MSU
Maintenance time
