摘要
[目的]研究9种植物精油及精油单体成分对黄瓜枯萎病的抑菌活性。[方法]采用滤纸片法和试管梯度稀释法测定了9种精油及精油单体成分对黄瓜枯萎病的抑菌活性。[结果]与阳性对照福美双相比,异硫氰酸丙烯酯和肉桂精油对黄瓜枯萎病的抑制作用较强,抑菌圈直径分别达(32.0±0.3)和(20.4±0.2)mm。异硫氰酸丙烯酯对黄瓜枯萎病的最小抑菌浓度(MIC)与最小杀菌浓度(MFC)均为0.049μL/mL,肉桂精油对黄瓜枯萎病菌的MIC和MFC均为0.098μL/mL,其余精油及精油单体的抑菌效果较弱。[结论]异硫氰酸丙烯酯和肉桂精油对黄瓜枯萎病的抑菌活性较好,具有进一步研究的价值。
[ Objective] The antifungal activity of nine essential oils and essential oil monomers was tested against Fusarium oxysporum f . sp. cucumerinum. [ Method] The antifungal activity of nine essential oils and essential oil monomers was studied by using filter paper-research and broth dilution method. [ Result] The inhibition zone of allyl isothiocyanate and cinnamon essential oil on Fusarium oxysporum f . sp. cucumeri- were （32. 0 ± 0. 3 ） and （20.4 ±0.2） mm, respectively, compared with the positive control. The minimal inhibitory concentration （MIC） and the minimal fungicidal concentration （MFC） on Fusarium oxysporum f . sp. cucumerinum of the allyl isothiocyanate were both 0. 049 （ jlL/mL, and the MIC and MFC on Fusarium oxysporum f . sp. cucumerinum of the cinnamon oil were both 0. 098 （jiL/mL. Their anti-fungal effect was close to thiram. But the inhibition effects of the rest of essential oils and essential oil monomers were weaker. [ Conclusion] Allyl isothiocyanate and cinnamon essential oil had better antifungal effects, which was important to further research and develop.
出处
《安徽农业科学》
CAS
2017年第31期160-162,181,共4页
Journal of Anhui Agricultural Sciences
基金
江苏省政策引导类计划(产学研合作)-前瞻性联合研究项目政策引导类计划项目(BY2016016-01)
国家自然科学青年基金项目(31501462)
江苏省自然科学青年基金项目(BK20150067)
关键词
植物精油
精油单体成分
黄瓜枯萎病
抑菌活性
Essential oil
Essential oil monomers
Fusarium oxysporum f . sp. cucumerinum
Antifungal activities
