摘要
[目的]探讨中国青凤蝶(Graphium sarpedon Linnaeus)3个亚种的遗传变异。[方法]对青凤蝶3个亚种的COⅠ基因和EF-1α基因部分序列进行测定,并对序列的碱基组成、转换颠换数和遗传距离等进行分析。[结果]在测得的COⅠ基因(709 bp)和EF-1α(1 064 bp)基因中,有44个变异位点,6个简约信息位点,COⅠ基因A+T平均含量为69.3%,存在较强的含量偏向性,亚种间的遗传距离相差甚小。[结论]青凤蝶3个亚种之间没有明显序列差异,仍然适宜采用传统的基于形态学的青凤蝶亚种分类体系。
[ Objective] To investigate the genetic variation in three subspecies of Graphium sarpedon Linnaeus in China. [ Method] The CO I gene and the EF-la gene were partially sequenced. The nucleotide composition, the number of transition and transversion, genetic distance of the segment had been analyzed. [ Result] There were 44 variation sites and 6 parsimony-informative sites in combined sequence of the CO I gene（709 bp）and EF-la（l 064 bp） gene in the examined species. The average A ＋ T content of CO I gene was 69. 3% , which showed strong A ＋ T bias. But genetic distance among subspecies were very small. [ Conclusion ] It has no significant sequence differences between the three subspecies, which is still suitable to use the traditional morphological classification system for Graphium sarpedon subspecies.
出处
《安徽农业科学》
CAS
2017年第31期156-159,共4页
Journal of Anhui Agricultural Sciences
基金
上海市金山区水务局专项"大金山岛猕猴容量调查研究"
上海科技馆种子资金项目
