摘要
目的:采用薄膜分散法制备鳖甲肽-长循环脂质体,并检测其表征。以超滤离心-HPLC法及透析法分别考察包封率及药物释放度。方法:采用薄膜分散法制备鳖甲肽-脂质体,检测粒径,PDI并观察脂质体电镜下形态。建立超滤离心-HPLC法测定方法并检测脂质体的包封率以筛选最佳药脂比;采用透析法考察药物在不同时间点的释放度。结果:采用超滤离心-HPLC法能有效测定鳖甲肽-脂质体的包封率,在药脂比1∶5时包封率最高。并且,在4 h时药物释放度最高,最后释放度趋于平稳。结论:本研究制备的脂质体粒径均匀性及形态良好,超滤离心-HPLC法可高效精确测定脂质体包封率,而透析法考察药物释放度稳定可靠,从而达到质量评价的目的。
Objective： To prepare HGRFG- SSL by thin- film dispersion method and detect its characterizions. The entrapment efficiency and drug release of HGRFG - SSL was determined by ultrafiltration centrifugation - HPLC method and the dialysis method, respectively. Methods ： The HGRFG - SSL was prepared by thin - film dispersion method. The particle size and PDI were detected and the morphology was observed under electron microscope. Establishing the uhrafiltration centrifugation - HPLC method and determining the entrapment efficiency were to screen the best drug - to - lipid ratio. The drug release was measured by dialysis method at different time points. Results： The encapsulation efficiency of HGRFG - SSL was effectively determined by uhrafihration centrifugation - HPLC method and the encapsulation efficiency was the highest at the drug - to - lipid ratio of 1 ： 5. The drug release rate reached the peak at 4 h and then almost tended to be stable. Conclusion： Ultrafihration centrifugation- HPLC method could accurately determine the encapsulation efficiency of liposomes, and dialysis method investigating the drug release was stable and reliable, which achieves the pur- pose of quality evaluation.
出处
《中华中医药学刊》
CAS
北大核心
2017年第11期2874-2877,共4页
Chinese Archives of Traditional Chinese Medicine
基金
国家自然科学基金项目(81573700)
浙江省自然科学基金项目(LY16H280004)
关键词
长循环脂质体
超滤离心-HPLC法
透析法
包封率
释放度
sterically stabilized liposomes
uhrafiltration centrifugation - HPLC
dialysis method
entrapment efficiency
drug release
