摘要
目的探讨长链非编码RNA（long non-coding RNA，lncRNA）-LOC391533与重度子痫前期胎盘螺旋动脉重铸不足的相关性。方法选择2016年1月至2016年12月在郑州大学第三附属医院住院的36例重度子痫前期孕妇为子痫前期组。按照1∶1的比例选择同期年龄相差2岁以内、孕周相差1周以内的产前检查正常的健康孕妇36例为对照组。采用扫描电子显微镜对2组孕妇胎盘组织中螺旋动脉管壁厚度及管腔面积进行测量。采用Western印迹法和酶联免疫吸附方法分别检测胎盘组织及母血血清中血管内皮细胞生长因子（vascular endothelial growth factor，VEGF）、可溶性VEGF受体-1（soluble VEGF receptor-1，sVEGFR-1）蛋白水平。采用逆转录-聚合酶链反应法检测胎盘组织中LOC391533 mRNA的含量。采用两独立样本t检验、Mann-Whitney U检验及Spearman相关分析进行统计学分析。结果（1）子痫前期组孕妇的平均管腔面积小于对照组[（130.1±22.3）与（188.1±21.5）μm2，t=10.888]，但平均管壁厚度较厚[（122.6±9.5）与（98.9±2.5）μm，t=－8.812]（P值均〈0.05）。（2）与对照组相比，子痫前期组胎盘组织和血清中sVEGFR-1蛋白水平均较高[胎盘组织：0.2±0.0与0.4±0.1，血清：（15.6±2.4）与（50.8±6.1） ng/L，t值分别为－17.569和－30.699，P值均〈0.05]，VEGF蛋白含量水平均较低[胎盘组织：0.6±0.1与0.2±0.0，血清：（40.8±3.2）与（28.1±3.2） ng/L，t值分别为18.013和16.200，P值均〈0.05]，胎盘组织中LOC391533 mRNA水平亦较高（1.0±0.2与2.4±0.5，t=－14.799，P〈0.05）。（3）子痫前期组胎盘组织中LOC391533 mRNA水平与螺旋动脉管壁厚度、胎盘组织及血清中sVEGFR-1蛋白呈正相关（r值分别为0.683、0.759及0.857，P值均〈0.05），与管腔面积、胎盘组织及血清中VEGF蛋白呈负相关（r值分别为－0.702、－0.806及－0.796，P值均〈0.05）。结论重度子痫前期患者胎盘及血清中VEGF及sVEGF-1的异常可能与螺旋动脉重铸不足的发生有关。
ObjectiveTo investigate the correlation between long non-coding RNA （lncRNA）-LOC391533 and inadequate placental spiral artery remodeling in severe preeclampsia （sPE）.MethodsThirty-six gravidas who were admitted to the Third Affiliated Hospital of Zhengzhou University with sPE from January 2016 to December 2016 were enrolled in sPE group. An equal number of healthy gravidas who experienced uneventful pregnancy and were of similar age （difference less than two years） and gestational age （difference less than one week） to those in the sPE group served as controls. Scanning electron microscopy was used to measure the luminal area and vessel wall thickness of placental spiral arteries for all gravidas. Levels of vascular endothelial growth factor （VEGF） and soluble VEGF receptor-1 （sVEGFR-1） in placenta tissues and maternal serum samples were detected by Western blot and ELISA. Reverse transcription-polymerase chain reaction （RT-PCR） was used to detect the expression of LOC391533 at mRNA level in placenta tissues of the two groups. Independent two samples t-test, Mann-Whitney U test and Spearman correlation analysis were used for statistical analysis.Results（1） The average luminal area of spiral arteries of the sPE group was smaller than that of the control group [（130.1±22.3） vs （188.1±21.5） μm2, t=10.888, P〈0.05], but the average thickness of spiral artery wall was thicker [（122.6±9.5） vs （98.9±2.5） μm, t=-8.812, P〈0.05]. （2） Compared with the control group, the sPE group showed increased sVEGFR-1 at protein level in both placenta tissues and serum samples [placenta： 0.2±0.0 vs 0.4±0.1, serum： （15.6±2.4） vs （50.8±6.1） ng/L, t=-17.569 and -30.699, both P〈0.05], decreased VEGF at protein level in both placenta tissues and serum samples [placenta： 0.6±0.1 vs 0.2±0.0, serum： （40.8±3.2） vs （28.1±3.2） ng/L, t=18.013 and 16.200, both P〈0.05], and enhanced expression of LOC391533 at mRNA level in placenta tissues （1.0±0.2 vs 2.4±0.5, t=-14.799, P〈0.05）. （3） Expression of LOC391533 at mRNA level in placenta tissues of the sPE group was positively correlated with spiral artery wall thickness and levels of sVEGFR-1 protein in placenta tissues and serum （r=0.683, 0.759 and 0.857, all P〈0.05）, and negatively correlated with luminal area and levels of VEGF protein in placenta tissues and serum （r=-0.702, -0.806 and -0.796, all P〈0.05）.ConclusionsAbnormal expression of VEGF and sVEGF-1 in placenta and serum of patients with sPE may be related to inadequate placental spiral artery remodeling.
出处
《中华围产医学杂志》
CAS
CSCD
2017年第10期718-723,共6页
Chinese Journal of Perinatal Medicine
关键词
先兆子痫
RNA
长链非编码
胎盘
血管内皮生长因子A
血管内皮生长因子
受体1
Pre-eclampsia
RNA, long noncoding
Placenta
Vascular endothelial growth factor A
Vascular endothelial growth factor receptor-1
