摘要
以安徽省霍山县漫水河百合健康新鲜鳞茎为研究对象,经过分离纯化筛选出一株内生菌株BF2,经菌落形态学和分子生物学鉴定BF2菌株属于散囊菌目踝节菌属Talaromyce;采用正交实验优化组合发酵,代谢产物通过滤纸片法得到对大肠杆菌与金黄色葡萄球菌最大抑制圈分别为3.05cm和3.26cm,最佳培养基组合为:NaNO30.6g;胰蛋白胨2g;葡萄糖25g和(NH4)2SO41.5g。
An endophytic strain named BF2 was isolated and purified from the healthy and fresh bulbs of the lily in the Manshuihe in Huoshan,Anhui province,and the strain was identified to belong to the Talaromyce genus of Eurotiales according to colonial morphology and Molecular biology methods. The optimum combination ferments were adopted using orthogonal experiment , and the maximum inhibition loops formed by the metabolites againtt E. coii and Staphylococcus aureus were 3. 05 cm and 3. 26 cm, respectively. The optimum medium combination was： NaNO3 , Tryptone, Glucose,and （NH4） SO4 were 0. 6 g ,2 g ,25g and 1. 5 g,respectively.
出处
《皖西学院学报》
2017年第5期10-14,共5页
Journal of West Anhui University
基金
安徽高校自然科学研究重点项目(KJ2017A403)
安徽高校自然科学研究重大项目(KJ2016SD61)
皖西学院质量工程项目(wxxy2017110
2016wxxy35
2016wxxy64)
皖西学院省级大学生创业创业训练项目(AH201510376027)联合资助
关键词
百合
内生菌
鉴定
lily
endophytes
identification
