摘要
目的探讨乙型肝炎病毒(Hepatitis B Virus,HBV)携带者孕产妇TGF-β1、IL-4、IFN-γ和IL-12的血清水平及胎盘中的表达,在HBV宫内感染中的意义。方法选择2011年1月-2012年11月间,在我院分娩的HBV感染孕妇86例作为研究对象,将其按照是否发生宫内感染分为阳性组和阴性组。采用酶联免疫吸附法对孕妇血清中TGF-β1、IL-4、IFN-γ和IL-12水平进行定量检测;采用免疫组化法对胎盘组织中TGF-β1、IL-4、IFN-γ和IL-12的表达水平进行检测。结果 (1)HBe Ag阳性组宫内感染发生率高于HBe Ag阴性组(P<0.05)。(2)宫内感染患者血清中TGF-β1和IL-4水平较未感染组明显升高(P<0.05),IFN-γ和IL-12水平较未感染组明显降低(P<0.05)。(3)TGF-β1、IL-4、IFN-γ和IL-12的阳性细胞主要定位在绒毛血管内皮细胞、胎盘滋养细胞和蜕膜细胞中,绒毛间质细胞表达量相对较少。表达的部位主要集中在细胞浆中。宫内感染组胎盘组织中TGF-β1和IL-4的阳性表达率高于未感染组(P<0.05);而IFN-γ和IL-12的阳性表达率则低于未感染组(P<0.05)。结论上述细胞因子的水平变化,反映了Th1/Th2细胞失衡,以及CD4^+CD25^+调节性T细胞(T regulatory cells,Treg)的过度活跃,这可能是HBV宫内感染发生的主要危险因素。孕妇HBeAg阳性预示HBV宫内感染率增加。
Objective To determine the serum level of TGF-β1,IL-4,IFN-γ and IL-12 from HBV carrier pregnant women,to investigate the expression of those cytokines in the placenta,and to analyze their relationship with HBV intrauterine infection.Methods The study recruited 86 HBV infected pregnant women in our hospital during Jan 2011-Nov 2012. The subjects were divided into 2 groups,intrauterine infected（＋） group and intrauterine uninfected（-） group based on the test results of neonates＇ HBV serum markers. Enzyme-linked immunosorbent assay（ELISA） was utilized to evaluate the serum levels of TGF-β1,IL-4,IFN-γ and IL-12. Immunohistochemistry（IHC） was utilized to identify the location and expression levels of TGF-β1,IL-4,IFN-γand IL-12 in the placenta. Results （1） The intrauterine infection rate was significantly higher in Hbe Ag positive group than in Hbe Ag negative group（P〈0.05）.（2）The serum levels of TGF-β1 and IL-4 in intrauterine infected（＋） group were significantly higher than those in intrauterine uninfected（-） group;while the serum levels of IFN-γ and IL-12 in intrauterine infected（＋） group were significantly lower than those in intrauterine uninfected（-） group（P〈0.05）. （3）The expression of TGF-β1,IL-4,IFN-γ and IL-12 mainly located in villous capillary endothelial cells,placental trophoblast cells and decidual cells,with a small amount of expression in villous stromal cells. The IHC positive rates of TGF-β1 and IL-4 in intrauterine infected（＋） group were significantly higher than those in intrauterine uninfected（-） group（P〈0.05）. The IHC positive rates of IFN-γ and IL-12 in intrauterine infected（＋）group were significantly lower than those in intrauterine uninfected（-） group（P〈0.05）. Conclusion Those cytokines＇ levels variation may reflect the imbalance between Th1 and Th2 cells and the hyperactive response of T regulatory cells,which might play a role in the HBV intrauterine infection. Maternal serum Hbe Ag positive could be used as a predictor of HBV intrauterine infection.
出处
《江西医药》
CAS
2017年第10期987-989,共3页
Jiangxi Medical Journal
基金
江西省科技厅科技计划项目
编号2012GZY0071
