摘要
目的探讨冠状动脉粥样硬化(atherosclerosis,AS)斑块稳定性与血清基质金属蛋白酶-9(matrix metallo-proteinase-9,MMP-9)及超敏C反应蛋白(high sensitivity C-reactive protein,hs-CRP)浓度的关系。方法按照随机分组方法将56只大白兔划分为2组:A组为AS研究组40只,A组分为A1亚组(斑块破裂组)、A2亚组(斑块未破裂组);B组为参照组16只。A组采用(标准颗粒+1%胆固醇)进行喂养+球囊损伤,B组采用标准颗粒喂养。在研究期间,动态监测A、B两组血清MMP-9和hs-CRP浓度及血脂的变化情况。结果研究期间,B组兔的体质量与A1、A2亚组比较,差异有统计学意义(P<0.05)。实验开始、实验第14周末,B组血脂指标变化不明显,A2和A1亚组的血脂指标由实验开始时到实验第14周末呈上升趋势;实验第14周末,B组与A1、A2亚组的血脂浓度比较,差异均有统计学意义(P<0.01);但是A2亚组和A1亚组血脂浓度比较,差异无统计学意义(P>0.05)。在第4周时,B组与A1亚组、A2亚组的血清MMP-9和hs-CRP浓度比较,差异有统计学意义(P<0.01)。在第10周时,B组、A2亚组和A1亚组的血清MMP-9和hs-CRP浓度比较,差异有统计学意义(P<0.01);A1亚组血清MMP-9和hs-CRP浓度高于A2亚组,且差异有统计学意义(P<0.01)。在第14周时,A1亚组和A2亚组的血清MMP-9和hs-CRP浓度均高于B组,差异有统计学意义(P<0.01);A1亚组血清MMP-9和hs-CRP浓度高于A2亚组,且差异有统计学意义(P<0.01)。实验过程中A1、A2两亚组的血清MMP-9、hs-CRP浓度均呈现升高趋势(P<0.01)。实验兔血清MMP-9浓度和hs-CRP浓度之间为正相关关系(r=0.967,P=0.001)。结论 MMP-9和hs-CRP呈正相关关系,且两者的浓度与AS斑块稳定性密切相关,斑块越不稳定,MMP-9和hs-CRP的浓度越高。
Objectives To evaluate and the relationships between coronary atherosclerosis（AS）plaque stability with serum concentrations of matrix metalloproteinase-9 （MMP-9） and high sensitivity C-reactive protein （hs-CRP）. Methods According to the random grouping method ,56 rabbits were divided into two groups：40 rabbits were in A group [including A1 subgroup （plaque broken） and A2 subgroup （plaque not broken）] which was the AS study group,16 rabbits were in B group which was a reference group. Rabbits were treated with standard particle feeding ＋1%cholesterol＋balloon injury in A group and rabbits were treated with standard particle feeding in B group. Serum con-centrations of MMP-9,hs-CRP and blood lipids were dynamicly monitored among the 3 groups. Results Differences of body weight of rabbits between B group with A1 subgroup and A2 subgroup were significantly different during the study（P〈0.05）. Blood lipids concentrations in B group were not significantly different but they were uptrend in A1 sub-group and A2 subgroup from the beginning to the 14th week in this study. Blood lipids concentrations in B group were significantly different with those of A1 subgroup and A2 subgroup（P〈0.05）at the 14th week in this study,but they had no statistic difference between A1 subgroup and A2 subgroup（P＆gt;0.05）. Serum concentrations of MMP-9 and hs-CRP in B group were significantly different compared with those in A1 subgroup and A2 subgroup in the 4th week（P〈0.05）. Differences of concentrations of MMP-9 and hs-CRP among B group,A1 subgroup and A2 subgroup were significant at the 10th week（P〈0.01）,and concentrations of MMP-9 and hs-CRP in A1 subgroup were obvious higher than those in A2 subgroup （P〈0.01）. Concentrations of MMP-9 and hs-CRP in A1 subgroup and A2 subgroup were significantly higher than those in B group at 14th week（P〈0.01）,and those in A1 subgroup were obviously higher than those in A2 subgroup（P〈0.01）. Concentrations of MMP-9 and hs-CRP in A1 subgroup and A2 subgroup were uptrend during this study. Concentration of MMP-9 positively related with concentration of hs-CRP （r=0.967,P=0.001）. Conclusions Concentration of MMP-9 positively relates with concentration of hs-CRP ,and they both closely relate to AS plaque sta-bility. The higher the concentrations of MMP-9 and hs-CRP are,the the more unstable the plaques are.
出处
《岭南心血管病杂志》
2017年第5期610-613,共4页
South China Journal of Cardiovascular Diseases
基金
贵州省科技计划课题(项目编号:黔科合SY字【2010】)两院合作项目
山东省自然科学基金项目(项目编号:ZR2010HM202)
