摘要
选取3种具有酚羟基的芳香类化合物,测定其对酪氨酸酶的活性影响。结果表明,1,5-二羟基萘在120~600μmol·L-1范围内对酪氨酸酶的激活活性>450%,最高为537%;2,2′-联苯酚的激活活性最大为284%;2,2′-联吡啶-3,3′二醇无激活活性。动力学研究表明,1,5-二羟基萘表现为非竞争性激活;2,2′-联苯酚为混合性激活作用,在酶促反应体系中,既影响底物与酶的结合,又改变酶的空间构型,综合提高反应速度。
Three phenolic hydroxyl compounds as activators of mushroom tyrosinase were ditermined.The results showed that the relative activity of tyrosinase was greater than 450%, the highest was 537%,when activated by 1,5-dihydroxynaphthalene at the range of 120-600 μmol·L-1. The activation effects of2,2′-biphenol was up to 284%, but 2,2′-bipyridine-3,3′-diol showed no obvious activation. The kinetic analysis showed that 1,5-dihydroxynaphthalene was a non-competitive activator; while 2,2′-biphenol was a mixed activator, which not only affected the combination of substrate and enzyme, but also changed the enzyme spatial configuration, comprehensively improved the response speed.
作者
黄天送
游遨
林耿鹏
劳思敏
马林
黎彧
HUANG Tian-song YOU Ao LIN Geng-peng LAO Si-min MA Lin LI Yu(Department of Environmental Engineering, Guangdong Industry Polytechnic, Guangzhou 510300, China School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, China)
出处
《药学与临床研究》
2017年第5期412-415,共4页
Pharmaceutical and Clinical Research
基金
广东省高等职业院校珠江学者岗位计划资助项目(2015)
广州市科技计划项目(201510010170)
广东轻院珠江学者人才类项目(RC2015-001)
广东省自然科学基金(2016A030313761)
广东省大学生科技创新培育专项资金项目(pdjh2016a0552)
中山大学生物无机与合成化学教育部重点实验室开放基金项目(2016)
广东轻工职业技术学院校级科研项目(KJPY003)
