摘要
采用3,5-二硝基水杨酸(DNS)法对菜用甘薯叶片还原糖和总糖的吸收光谱、测定和提取条件进行研究,以间接测定多糖含量。结果表明,菜用甘薯叶片中还原糖的最适检测波长为493 nm,DNS显色剂用量为2.0 mL,显色反应时间为10 min,显色反应后静置时间10 min。还原糖及总糖样品液提取水浴温度为100℃,水浴时间为30 min;总糖样液酸解时盐酸用量为2.0 mL,沸水浴时间为20 min。此方法精密度、重现性和回收率符合实验要求,可准确测定菜用甘薯叶片中的多糖含量,实现了对菜用甘薯叶片中还原糖、总糖和多糖含量的同时测定。
The reducing sugar and total sugar contents of the leaves of vegetable sweet potato （Dioscorea esculenta （Lour.） Burkill） were determined by using the 3＇5＇-dinitrosalicylic acid （DNS） method to indirectly determine the leaf content of polysaccharides of three vegetable sweet potato varieties. The optimum conditions for the deter- mination of reducing sugar and total contents were as follows ： Optimum wavelength 493 nm, DNS reagent 2.0 mL, coloring reactionl0 min, and setting time 10 min. For the reducing sugar and total sugar, the best extraction conditions would be boiling water bath for 30 min. The total sugar was hydrolyzed with hydrochloric acid at a rate of 2.0 mL, and extracted in boiling water bath for 20 min. With this method the polysaccharide contents of the leaves of the three vegetable sweet potato varieties were 12.63, 13.49 and 18.51 mg ~ g-1 FW respectively. The repeatability and recovery of this method meet the test requirements. It is concluded that this method can be used for the determination of polysaccharide content in vegetable sweet potato leaves, and can also be used for the determination of reducing sugar and total sugar.
出处
《热带生物学报》
2017年第3期359-363,共5页
Journal of Tropical Biology
基金
基金项目:热作无公害生产技术集成与示范推广(15RZNJ-57)
优质富硒菜用甘薯周年生产技术示范与推广(HNXH201530)
海南富硒地区甘薯硒的富集能力
贮存形态及分布规律研究(20163067)
