摘要
以氮高效(H2000-03)和氮低效(册亨家麻)2个基因型苎麻品种为材料,设置氮素水平(0、6、9、12、15 mmol/L)开展温室盆栽试验,研究氮素调控苎麻纤维发育关键酶的生理机制。结果表明,苎麻蔗糖合成酶(SS)、β-1,3-葡聚糖酶和吲哚乙酸氧化酶(IAAO)活性随氮素水平提高呈现逐渐增加的趋势,并在超过9 mmol/L后增幅显著下降。各酶活性随生长发育时期均呈单峰变化趋势,表现为旺长期>幼苗期>成熟期。氮高效基因型的相关酶活性均高于氮低效基因型,且SS和β-1,3-葡聚糖酶活性在幼苗期和旺长期达到显著水平,IAAO活性在旺长期和成熟期达到显著水平(p<0.05)。相关酶对苎麻原麻产量具有重要影响,不同基因型对其响应特征不同,其中氮高效基因型的响应较敏感。苎麻纤维发育存在适氮水平,氮高效基因型可耐受更高氮素水平,并表现出显著较高的生产力和稳产性。
Two ramie varieties,H2000-03 with average nitrogen utilization efficiency is 46.01%and Ceheng Jiama,with average nitrogen utilization efficiency 15.81%,were used to perform potted experiment,with 5 nitrogen application levels （0 mmol/L,6 mmol/L,9 mmol/L,12 mmol/L,15 mmol/L）,under glass in Changsha,Hunan Province.Enzyme activities related to fiber development were analyzed for understanding their physiological mechanism under different nitrogen levels.The results showed that： Sucrose synthetase （SS）,β-1,3-Glucanase and IAAO activities increased along with the increasing of nitrogen application.The increasing rate slowed down when nitrogen amount exceeded9 mmol/L.Every enzyme activity showed a single-peak curve along with the growth periods of ramie and the sequence from high to low was fast growing period,seedling period and maturing period.Enzyme activities of H2000-03,which had a higher nitrogen utilization efficiency,was higher than Ceheng Jiama,especially for SS and β-1,3-Glucanase at early period （seedling and fast growing period）,for IAAO at later period （fast growing and maturing period） （p 0.05）.Raw fiber yield of ramie was significantly affected by enzyme activities and showed different responding characteristics between varieties.Ramie variety with high nitrogen utilization efficiency was more sensitive to enzyme.There was an optimum range of nitrogen for fiber development of ramie.Ramie variety with high nitrogen utilization was better tolerated to higher nitrogen levels and represented higher productivity and yield stability.
出处
《中国麻业科学》
2017年第5期257-263,共7页
Plant Fiber Sciences in China
基金
中国农业科学院基本科研业务费专项资助(1610242016027)
国家麻类产业技术体系建设专项资助(CARS-19)
关键词
苎麻
氮效率
纤维
酶
氮素水平
ramie
nitrogen utilization efficiency
fiber development
enzyme
nitrogen levels
