摘要
碱性条件下,丹参酮ⅡA磺酸钠能够抑制鲁米诺-过氧化氢-银纳米粒子的化学发光,据此建立了测定丹参酮ⅡA磺酸钠的化学发光分析方法。通过优化发光底物浓度、反应介质及其浓度和银纳米粒子浓度等因素,确立了最佳实验条件,并探讨了体系的可能机理。在5.0×10^(-6)~0.08 mol/L浓度范围内,体系的化学发光强度与丹参酮ⅡA磺酸钠浓度的对数值呈线性关系,检出限为9.38×10^(-7)mol/L,检测时间为20 s。该方法灵敏度好,检测范围宽,可简单快速地测定注射液中丹参酮ⅡA磺酸钠的含量,检测结果与高效液相色谱法的测定结果较为一致。
A novel method for the detection of sodium tanshinon ⅡA sulfonate was developed based on the inhibition effect of sodium tanshinon ⅡA sulfonate on luminol - H2O2 - silver nanoparticles （AgNPs） chemiluminescence（CL） under alkaline condition. The optimal experiment conditions were established by optimizing the concentration of CL substrates, reaction medium and its concentration, and the concentration of AgNPs. The possible mechanism of the CL system was also investigated. Under the optimal conditions, the CL intensity was inversely proportional to logarithm value of sodium tanshinon ⅡA sulfonate concentration in the range of 5.0 × 10-6 - 0.08 mol/L with a detection limit of 9. 38 × 10 -7 mol/L and a detection time of 20 s. With a considerable sensitivity and a wide detection range, the proposed method could be applied in the simple and fast detection of sodium tanshinon Ⅱ A sulfonate in the injection solution with satisfactory results which are in good agreement with the reference values obtained by high performance liquid chromatography.
出处
《分析测试学报》
CAS
CSCD
北大核心
2017年第10期1245-1249,共5页
Journal of Instrumental Analysis
基金
国家自然科学基金青年基金(21505160)
关键词
化学发光
银纳米粒子
鲁米诺
丹参酮ⅡA磺酸钠
chemiluminescence
silver nanoparticles
luminol
sodium tanshinon ⅡA sulfonate
