摘要
目的 探讨黏附分子CD4 4、上皮性钙黏附蛋白 (E cad)和转移抑制基因nm2 3 H1与甲状腺滤泡源性癌分化、浸润转移的关系。方法 采用免疫组织化学SP法和EnVision法检测 4 2例滤泡癌和 5 4例乳头状癌中CD4 4、E cad和nm2 3 H1的表达。结果 CD4 4主要表达于癌细胞及浸润淋巴细胞胞膜 ,低分化滤泡癌和有转移乳头状癌CD4 4表达分别高于高分化滤泡癌和无转移乳头状癌。E cad阳性物质和nm2 3 H1基因产物均分布于癌细胞胞质 ,表达程度与滤泡癌分化呈正相关 ,乳头状癌的E cad和nm2 3 H1阳性率高于滤泡癌 ,转移癌的阳性率和表达强度低于原发灶。甲状腺滤泡源性癌CD4 4检测阳性率高于E cad和nm2 3 H1。在滤泡癌中E cad与nm2 3 H1的表达之间呈正相关关系 ,而在乳头状癌中呈正相关趋势。CD4 4与E cad的表达、CD4 4与nm2 3 H1的表达之间在滤泡癌和乳头状癌均呈负相关趋势。结论 综合检测CD4 4、E cad和nm2 3对甲状腺滤泡源性癌的诊断。
Objective To investigate the relationship between expression of cell adhesion molecular CD44, epithelial cadherin (E cad) and metastastic suppressor gene nm23 H1 as well as the clinicopathologic features including cell differentiation, invasion and metastasis of thyroid follicular derived carcinoma Methods Forty two (42) thyroid follicular carcinomas (FTC) and 54 papillary carcinomas (PTC) were collected for studying the expression of CD44, E cad and nm23 H1 using immunohistochemical staining Results Neoplastic epithelium and infiltrating lymphocyte expressed CD44 in an intense plasma membrane pattern CD44 expression rates in poorly differentiated FTC (80%) and PTC cases with metastasis (78%) were significantly higher than those of well differentiated FTC cases (64%) and PTC without metastasis cases (59%) respectively Thyroid carcinoma tissue was positive for E cad and nm23 H1 in a cytoplasm pattern Well differentiated FTC presented a higher E cad and nm23 H1 expression rate than poorly differentiated FTC, but both had a lower expression rate than that of PTC (70% and 76%, P <0 01) The expression rate and intensity of E cad and nm23 H1 were lower and less in metastastic PTC than those in primary PTC Expression rate of CD44 (72%) in thyroid follicular derived carcinoma was higher than those of E cad (54%, P <0 01) and nm23 H1 (61%, P <0 05) E cad expression was adversely correlated with that of nm23 H1 (χ 2= 15 75, P <0 011, r =0 522 2) There was a reverse relationship between expression of CD44 and E cad or nm23 ( P >0 05) Conclusions Cell differentiation degree in FTC and metastasis in PTC have positive correlation with the expression of E cad and nm23, but have a reverse correlation with the expression of CD44 There was a relationship between expression of CD44, E cad, nm23 and the characteristics of the degree of differentiation, metastastic potential and the prognosis of thyroid follicular derived carcinoma
出处
《中华病理学杂志》
CAS
CSCD
北大核心
2002年第4期322-326,共5页
Chinese Journal of Pathology
基金
天津市科学技术委员会科学研究基金资助项目(0 0 3 60 5 811)
