摘要
普萘洛尔是常用的β受体阻断剂,对心律失常、心绞痛、高血压、偏头痛等心血管疾病具有有效的预防和治疗作用,目前国内临床上仅用普通片剂,而国外尚有控释片、控释胶囊等剂型。普萘洛尔的HPLC-UV法检测曾有报道。
A method of HPLC assay with fluorescence detector was developed for determining propranolol concentration in plasma. The exprimental conditions were: mobil phase, methanol-0.035% acetate buffer solution (80:20 V/V) mixture; column, Zorbox-CN(250 4. 6 mm id); fluorescence detector, EX 285 nm and EM 345 nm; extraction solvent, ether. The average recovery of propranolol was 96.5%. The coefficent of variation was less than 5.5%. The method is simple, rapid, sensitive and repetitive, Its linear range is 5-100 ng/ml (r=0.9998). The method can be used for propranolol assay and kinetics study.
出处
《中国药科大学学报》
CAS
CSCD
北大核心
1991年第2期111-113,共3页
Journal of China Pharmaceutical University
关键词
高效液相色谱
普萘洛尔
血浆
HPLC
Fluoresence detector
Propranolol
Plasma
