摘要
本研究基于聚醚醚酮的骨科植入材料生物相容性的研究,进一步分析聚醚醚酮(polyether-ether-ketone,PEEK)骨科植入材料生物的相容性能,以有效提高其生物功能性,进而获得更加可靠的骨-植入体界面。本研究通过以新西兰大白兔为研究对象,采用改性/未改性的PEEK材料制备了腰椎模型并植入,最后进行了生物力学与Micro CT分析。实验研究表明,以改性及未改性的PEEK材料制备的腰椎假体在植入动物体内后后,动物体内未发生显著相关的急慢性毒性反应。对比植入的两种PEEK假体我们发现,改性的PEEK腰椎假体在植入动物后第4周,其临近椎骨的植入部位在骨体积分数等参数上发生了明显的改善(p<0.05);植入后第8周检查,以骨体积分数(BV/TV)、(骨密度(BMD)、骨小梁厚度(Tb.Th)、骨小梁分离度(Tb.Sp)以及骨表面体积分数(BS/BV)等为代表的多项重要的骨组织形态计量学参数均发生了显著的改善(p<0.05)。同时,研究通过采用拔出试验来验证植入材料的生物力学性能,实验发现改性PEEK材料能够有效促进植入假体与椎骨的紧密结合。因此,实验中针对PEEK材料采用rh BMP-2与胶原蛋白对表面进行改性可以高效地、快速地达到效果,并且能够明显提高骨科植入的PEEK材料的生物功能性与生物相容性。
Based on polyether ether ketone orthopaedic implant materials biocompatibility studies,this study aimed to further analyze the biological compatibility performance of polyether-ether-ketone（PEEK） as orthopaedic implant materials,so as to improve its biological function,and then get more reliable bone-implant interface.New Zealand white rabbits were chosen as the research objects,and implanted with lumbar spine model prepared by modified or unmodified PEEK material,which were then tested by biomechanics and Micro-CT methods.It was shown that no acute and chronic toxicity were observed in experimental animals after implanted with lumbar prosthesis prepared by modified or unmodified PEEK materials.Compared the two kinks of implanted PEEK materials,we found that 4 weeks after implantation of modified PEEK prosthesis,the implanted section near the lumbar had a significant improvement,especially in bone volume fraction（p〈0.05）;after 8 weeks,improvements were observed in several morphometric parameter of bone tissue,including bone volume fraction（BV/TV）,bone mineral density（BMD）,bone trabecular thickness（Tb.Th）,trabecular bone isolation（Tb.Sp） and bone surface volume fraction（BS/BV）.At the meantime,the pull-out test was introduced to analyze the biomechanical property of implanted materials,the result of which indicated that the modified PEEK prosthesis materials could promotethe integration of lumbar spine and prosthesis.Therefore,a PEEK materials treated with rh BMP-2 and collagen protein on the surface could carry out the effect much quicker and more effective,and could obviously improve the biological function and biocompatibility of orthopaedic implanted PEEK materials.
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2017年第9期3564-3571,共8页
Genomics and Applied Biology
基金
新疆医科大学附属中医医院资助
关键词
骨科植入材料
聚醚醚酮
生物相容性
表面改性
Orthopaedics embedded material
PEEK
Biocompatibility
Surface modification
