摘要
目的:比较慢性鼻-鼻窦炎伴鼻息肉(CRSwNP)及慢性鼻-鼻窦炎不伴鼻息肉(CRSsNP)患者鼻腔金黄色葡萄球菌携带毒力基因的差异,探索不同毒力因子与鼻窦炎、鼻息肉发病之间的关系。方法:以71例CRS患者为实验组(CRSwNP患者33例,CRSsNP患者38例),同期103例非CRS者为对照组。采用PCR方法对从鼻腔内分泌物中分离鉴定的41株金葡菌所携带的17种毒力基因进行检测。结果:①实验组seb检出率明显高于对照组(χ~2=5.864,P<0.05);CRSwNP组中seb检出率高于CRSsNP组(P<0.05);其余5种肠毒素基因实验组与对照组比较均差异无统计学意义(P>0.05);②金葡菌黏附素在实验组及对照组中均有检出,且检出率都较高,比较实验组和对照组检出率时,差异无统计学意义(P>0.05);③编码溶血素的HLα、HLβ基因在实验组和对照组中检出率都较高,且这两种基因在实验组与对照组中比较均差异无统计学意义(P>0.05)。结论:携带有肠毒素基因的金葡菌更易引起CRS,且可能携带肠毒素种类越多的菌株感染与鼻息肉的发病关系越大。
Objective：To investigate the differences of virulence genes of Staphylococcus aureus in patients with chronic sinusitis with nasal polyps（CRSwNP）and chronic sinusitis without nasal polyps（CRSsNP）,and to explore the relationship between different virulence factors and the incidence of sinusitis and nasal polyps.Method：The PCR method was used to detect 17 kinds of virulence genes of Staphylococcus aureus isolated from nasal secretions of 41 strains.Result：①The detection rate of SEB in the experimental group was significantly higher than that in the control group,the detection rate of SEB in the CRSwNP group was higher than that in the CRSsNP group,and the other 5 kinds of enterotoxin genes were not statistically significant between the experimental group and the control group;②Staphylococcus aureus adhesin in the experimental group and the control group were detected,and the detection rate was relative higher in both control and experimental goups.However,the detection rate were not statistically significant between the experimental group and the control group;③The detection rate of HLαand HLβgenes encoding hemolysin was higher in the experimental group and the control group,and there was no significant difference between the 2 groups in the experimental group and the control group.Conclusion：Staphylococcus aureus carrying enterotoxin gene was more likely to cause CRS.The greater the incidence of nasal polyps was found in patients infected by Staphylococcus aureus carrying more enterotoxin gene.
出处
《临床耳鼻咽喉头颈外科杂志》
CAS
北大核心
2017年第18期1393-1397,共5页
Journal of Clinical Otorhinolaryngology Head And Neck Surgery
