摘要
GID1是赤霉素信号通路关键调控基因,其通路主要影响种子萌发和植物生长。本文以高通量测序构建的蚬壳花椒(Zanthoxylum dissitum)cDNA文库为参考,克隆了蚬壳花椒3个GID1同源基因ZaGID1a、ZaGID1b、ZaGID1c。利用生物信息学对蚬壳花椒GID1同源基因的核酸序列及氨基酸序列进行分析,表明3个同源GID1序列编码的蛋白功能区域、理化性质、系统发育、亲疏水性、亚细胞信号肽定位、三级结构预测等各项生物信息学信息均指向赤霉素受体蛋白功能。同源GID1序列在蚬壳花椒种子人工萌发过程中的实时荧光定量PCR(qRT-PCR)表达量分析研究表明,ZaGID1a、ZaGID1c可能对蚬壳花椒种子萌发起关键调控作用,ZaGID1b与种子萌发过程的关系不明显。
GID1 is the key regulatory gene in gibberellin signaling pathway, which mainly affects seed germination and plant growth. In this study, we cloned three homologous genes of GID1 in Zanthoxylum dissitum, namely ZaGID1 a, ZaGID1b and ZaGID1c, by referring to its DNA library established by high throughput sequencing. Bioinformatics was applied to analyze nucleotide and putative amino acid sequences of homologous genes of GID1 in Z. dissitum, and the results indicate the encoded protein functional region, physiochemical property, phylogeny, hydrophobicity-hydrophilicity, subcellular signal peptide location and tertiary structure prediction in three homologous GID1 sequences point to the receptor protein function of gibberellin. Quantitative real time PCR expression analysis of homologous GID1 sequences via Z. dissitum artificial germination indicates ZaGID1 a and ZaGID1c played a critical role in the regulation of Z. dissitum seed germination, while the relation between ZaGID1b and seed germination was not evident.
出处
《植物生理学报》
CAS
CSCD
北大核心
2017年第8期1499-1506,共8页
Plant Physiology Journal
基金
国家自然科学基金(31370612)
湖南省高校科技成果产业化繁育项目(15CY011)~~
关键词
蚬壳花椒
克隆
表达分析
生物信息学
Zanthoxylum dissitum
cloning
expression analysis
bioinformatics
