摘要
目的:探讨川芎提取液对D-半乳糖致衰老小鼠学习记忆的影响及其与血清、脑、肝脏、肾脏等组织中SOD活力、GSH-Px活性和MDA含量的关系。方法:采用小鼠颈背部皮下连续注射D-半乳糖90d建立衰老小鼠模型,衰老模型成功的小鼠分为3组:衰老模型组、生理盐水对照组、川芎提取液组,每组8只,川芎提取液组按12g/kg连续灌胃14d,生理盐水对照组灌与川芎治疗组相同容量的生理盐水,衰老模型组小鼠不灌任何东西。另8只小鼠不注射D-半乳糖作为正常对照组。Y型迷宫实验测试小鼠学习记忆能力;对小鼠血清、脑、肝脏、肾脏采样,测定其中超氧化物歧化酶(SOD)活力、谷胱甘肽过氧化物酶(GSH-Px)活性和丙二醛(MDA)含量。结果:与正常对照组相比,衰老模型组学习测试次数和记忆错误次数均明显增多;与衰老模型组相比,川芎提取液组学习测试次数和记忆错误次数均明显减少,差异均有统计学意义(P<0.05)。与正常对照组相比,衰老模型组血清、脑、肝、肾脏SOD活力和GSH-Px活性均明显降低而MDA含量均明显升高;与衰老模型组相比,川芎提取液组血清、脑、肝、肾脏SOD活力和GSH-Px活性均明显升高;而MDA含量均明显降低,差异均有统计学意义(P<0.05)。结论:川芎可能通过升高血清、脑、肝、肾脏SOD活力和GSH-Px活性,降低MDA含量,从而改善衰老小鼠学习记忆能力。
Objective：To explore the effect of Ligusticum chuanxiong hort abstract to the ability ot learning and memory and the levels of SOD, GSH-Px and MDA in serum, brain, liver and kidney in aging mice treated with D-galactose. Methods： Made aging mice with D-galactose for 90 days. Divided aging mice into 3 groups： aging mice model group, saline group and Ligustieum chuanxiong hort abstract group. Each group had 8 mice. Ligusticum chuanxiong hort abstract group was treated Ligusticum chuanxiong hort abstract 12g/kg for 14 days. The control group was clear with Dgalactose. The spatial learning and memory ability were evaluated by Y-maze. Mice＇s serum, brain, liver and kidney were collected and the activity of SOD and GSH-Px, the level of MDA were measured. Results：Compared with the con- trol group, the learning ability of aging mice group were significantly increased （P〈0. 05） ,the memory ability of Ligusticum chuanxiong hort abstract group was significantly increased （P〈0. 05） ;the activity of SOD, GSH-Px in serum, brain, liver and kidney decreased significantly in aging mice group compared with the control roup（P〈0. 05） ; the activity of MDA in serum, liver,kidney and heart increased significantly in aging mice group compared with the control group（P〈0. 05）. the activity of SOD, GSH-Px in serum, brain, liver and kidney increased significantly in Ligusticum chuanxiong hort abstract group compared with the control group（P〈0.05） ;the activity of MDA in serum, brain, liver and kidney decreased significantly in aging mice group compared with the control group（P〈0. 05）. Conclusion： Ligusticum chuanxiong hort increases the activity of SOD and GSH-Px, decreases the level of MDA in mice＇ s serum,brain, liver and kidney and improves the ability of learning and memory.
出处
《医学理论与实践》
2017年第18期2661-2663,2666,共4页
The Journal of Medical Theory and Practice
基金
右江民族医学院大学生创新创业训练计划项目(XJCXA201418)
关键词
川芎
衰老小鼠
学习记忆
超氧化物歧化酶
谷胱甘肽
丙二醛
Ligusticum chuanxiong hort, Aging mice, Learning and memory, Superoxide dismutase, Glutothine perox-idase, Malondialdehyde
