摘要
盐渍化是危害大豆生产的主要非生物胁迫因素之一。目前大豆耐盐性研究主要集中在栽培大豆的苗期耐盐性,而芽期耐盐性状的研究相对较少。野生大豆蕴含丰富的耐逆基因,是栽培大豆遗传改良的重要资源。为了研究野生大豆芽期耐盐性状的遗传机制,以113份野生大豆为试验材料,进行芽期耐盐性状的鉴定,结合群体的分子标记对包括2年平均值在内的3个环境下的3个耐盐指数进行全基因组关联分析,共检测到与野生大豆芽期耐盐相关的位点26个,6个SSR标记Satt521、Satt022、Satt239、Satt516、Satt251和Satt285在2个或3个环境下均被检测到,4个SSR标记Satt516、Satt251、Satt285和GMES4990与2个或3个耐盐指数显著相关。对这些SSR标记进行分析,挖掘了最优的等位基因及其载体材料。以上这些结果对于阐明野生大豆芽期耐盐性状的遗传机制,进一步发掘新的耐盐基因具有重要意义。同时也为栽培大豆遗传基础的拓宽、大豆耐盐分子标记辅助选择和分子设计育种等后续研究提供重要依据。
Salinity is one of major abiotic stress factors threatening soybean production. The previous studies mainly focused on salt tolerance at the soybean seedling stage. A few studies regarding salt tolerance during germination have been reported. Wild soybeans with richness of resistance genes are valuable resources for soybean genetic improvement. The objective of this study was to identify the genetic mechanisms of wild soybean seed germination under salt stress. One natural population con- sisting of 113 wild soybean accessions was used in this study. Three salt tolerance indices were measured. The molecular markers associated with salt tolerance were detected in 2013, 2014 and across two years by combining SSRs of genome-wide association analysis. 26 SSR-trait associations were identified using a mixed linear model and the TASSEL 2. 1 software. Six SSRs, Satt521, Satt022, Satt239, SattS16, Satt251 and Satt285, were identified in two or three environments. Four SSRs, Satt516, Satt251, Satt285 and GMES4990, were co-associated with two or three salt tolerance indices. Furthermore, elite al- leles and their carrier materials were identified by analyzing alleles at the above loci. These results could contribute to eluci- date the genetic mechanisms of salt tolerance, explore new salt tolerant genes, and provide important foundation for broadening soybean＇ s genetic base, molecular marker assisted selection and molecular design breeding in soybean salt tolerance breeding.
出处
《大豆科学》
CAS
CSCD
北大核心
2017年第5期737-745,共9页
Soybean Science
基金
中央高校基本科研业务费专项资金(KYZ201705)
关键词
野生大豆
芽期
耐盐
关联分析
Wild soybean
Germination
Salt tolerance
Genome-wide association mapping
