摘要
目的观察Runx2在人退变腰椎间盘软骨终板中的表达情况并探讨其与软骨终板退变的关系。方法收集人腰椎间盘软骨终板组织42例,其中,椎体骨折患者的手术标本20例为对照组,椎间盘退变患者的手术标本22例为实验组。H-E染色、免疫组织化学法、及PCR法检测Runx2的表达量;Western印迹法检测两组之间Runx2、aggrecan及collagen I的表达量。结果对照组终板软骨结构清晰完整,而退变组的软骨终板结构破坏严重。正常终板软骨中几乎没有Runx2的表达,退变的软骨终板可见明显的Runx2阳性细胞。轻度退变组、中度退变组和重度退变组Runx2 mRNA的表达量分别为对照组的(4.74±0.78)、(7.74±0.97)、(16.97±1.89)倍。Runx2 mRNA的表达量与腰椎间盘软骨终板退变的程度呈明显的正相关。Western印迹法显示,轻度退变组Runx2、aggrecan及collagen I蛋白表达量分别为对照组的(2.79±0.52)倍、(0.48±0.12)、(0.67±0.17)差异有统计学意义(均P<0.05);中度退变组Runx2、aggrecan及collagen I蛋白表达量分别为对照组的(4.40±1.78)倍、(0.23±0.14)、(0.36±0.12)差异有统计学意义(均P<0.01);重度退变组Runx2、aggrecan及collagen I蛋白表达量分别为对照组的(8.75±1.88)倍、(0.13±0.09)、(0.08±0.03)差异有统计学意义(均P<0.01)。结论 Runx2可以作为预测腰椎间盘退变和软骨终板退变严重程度的指标,并可作为治疗腰椎间盘退变性疾病的潜在靶点。
Objective To investigate the expression o f Runx2 in degenerated lumbar endplate cartilage and its clinical significance. Methods Twenty two specimens o f degenerated human lumbar cartilage endplates （study group） and 20 specimens o f non-degenerated lumbar cartilage endplate （control group） were collected. H-E staining was used to examine the morphological changes o f lumbar cartilage endplate. The expression o f Runx2 protein and mRNA was detected with inmiunohistcjchemistry, RT-PCR and Western blo t t ing, respectively ; the expression o f aggrecan and collagen I was also detected with Western blotting in two groups. Results The structure o f cartilage endplate was complete and compactly arranged in control group, while in the degenerated group the structure o f endplate was seriously destructed. Immunohistochemistry analysis showed that the Runx2-positive cells were scarcely distributed in normal endplates, while significantly widely distributed in the degenerated endplates. In the mi ld, moderate and severe degenerative groups, RT-PCR analysis showed that the mRNA expression of Runx2 was p o sitiv e ly correlated with the degenerative degree o f endplate cartilage, which was （4. 74 ± 0.78 ）,（7. 74 ± 0. 97） and （16. 97 ± 1. 89） fo ld higher than that o f control group, respectively. Western-blotting showed that the expressions o f Runx2 , aggrecan and collagen I protein in mi ld,moderate and severe degenerative group were （2. 79 ± 0. 5 2 ）, （0. 48 ± 0. 12） , （0.67 ±0. 17） fold （P 〈0.05） ; （0.36 ±0. 12）, （0.23 ±0. 14）, （4.40 ±1.78） fold （ P 〈0.01） ; and （8. 75 ± 1. 88）,（0. 13 ±0. 09）,（0. 08 ±0. 03） fo ld （P 〈0.01） higher than those in control group. Conclusion Runx2 can be an ideal indicator to predict the degeneration degree o f lumbar endplate cartilage and may serve as a potential treatment target for lumbar disc degenerative diseases.
出处
《同济大学学报（医学版）》
CAS
2017年第4期21-25,共5页
Journal of Tongji University(Medical Science)
基金
上海市卫生与计划委员会科研项目(201640063)
江西省自然科学基金项目(20171BAB205034)
