摘要
目的通过研究婴儿痉挛症(IS)的神经内分泌免疫网络功能,探讨神经内分泌免疫网络在IS发病机制中的作用。方法共收集30例符合标准IS病例(IS组)与30例健康婴幼儿(对照组),采用化学发光法检测血清皮质醇、ACTH、IGF1、IL1B、IL2R、IL6、IL8、TNFα;利用酶联免疫吸附法检测血清CRH;免疫比浊法检测血清免疫球蛋白IgA、IgM、IgG;采用流式细胞术检测T细胞亚群(CD3+、CD4+、CD8+)的比例。结果 IS组血清CRH、IL2R、IL8、TNFα水平均较正常对照组高,且有统计学意义(P<0.05),而IS组血皮质醇、ACTH、IGF1、IL1B、IL6、IgA、IgM、IgG、(CD3+、CD4+、CD8+)所占比例、CD4+/CD8+比值与正常对照组比较无明显差异(P>0.05);平均每日发作串数分别与CRH、IL2R、IL8、TNFα呈正相关(P<0.05),平均每日发作总次数与CRH、IL8呈正相关(P<0.05),且CRH、IL2R、IL8、TNFα两两间也呈正相关(P<0.05)。结论 IS的发病机制比较复杂,与神经内分泌免疫网络有关,提示免疫炎症发病机制在IS的发生发展中可能有着重要作用。
Objective To explore the role of neuroendocrine immune network in pathogenesis of infantile spasms（IS）. Methods A total of 30 patients with IS（IS group） and 30 cases of healthy infants and young children（control group） were recruited in this study. Serum cortisol,ACTH,IGF1,IL1 B,IL2R,IL6 and IL8 and TNFα were determined by the chemiluminescence analysis,serum CRH was measured by enzyme-linked immunosorbent assay,serum immunoglobulin Ig A,Ig M and Ig G were detected by Immunoturbidimetric assay and T cell subsets including CD3 ＋,CD4 ＋ and CD8 ＋ were tested by flow cytometry. Results serum CRH,IL2 R,IL8 and TNFα levels of IS group was significantly higher than normal control group（P〈0.05）. serum cortisol,ACTH,IGF1,IL1 B,IL6,Ig A,Ig M and Ig G,proportion of CD3＋,CD4＋,CD8＋,and CD4＋/CD8＋ ratio in IS group were no evident differences compared with control group（P〉0.05）. The average number of daily ictal clusters positively correlated with CRH,IL2 R,IL8and TNF alpha respectively. The average daily total seizure number positively correlated with CRH,IL8 respectively. Meanwhile,there were also positively correlated each other between CRH,IL2 R,IL8 and TNF alpha. Conclusion Neuroendocrine immune network involved in the pathogenesis of IS,for which change of immune function,especially inflammation,plays a important role.
出处
《江西医药》
CAS
2017年第8期733-737,745,共6页
Jiangxi Medical Journal
基金
江西省卫生计生委课题
编号20161119
