摘要
目的:建立生脉饮(红参、五味子、麦冬)中五味子甲素、五味子乙素、五味子丙素、五味子醇甲、五味子醇乙、五味子酯甲、五味子酚的含量测定方法。方法:采用超高效液相色谱-质谱联用(UPLC-MS)法进行定量分析,色谱柱ACQUITY UPLC BEH C18(2.1 mm×50 mm,1.7μm),以乙腈(A)-0.1%甲酸水溶液(B)为流动相梯度洗脱:1~5 min,A-B(40∶60);5~7 min A-B(40∶60~70∶30);7~10 min A-B(70∶30);10~11 min 100%A;11~12 min A-B(40∶60),流速为0.3 mL·min^(-1),进样量2μL,柱温30℃,采用ESI离子源在正离子模式下选择离子扫描模式进行定量分析。结果:7种木脂素在一定范围内线性良好,相关系数均大于0.999,加样回收率在95.15%~100.84%之间。结论:该方法快速、灵敏度高、选择性好,适合测定生脉饮中木脂素的含量。
OBJECTIVE To develop a new method to determine deoxychisandrin,schisandrin B,schisandrin C schisandrin,schisandrol B,schisantherrin A and schisanhenol in Shengmaiyin.METHODS Seven lignans were determined by UPLC-MS.ACQUITY UPLC BEH C18column（2.1 mm×50 mm,1.7μm）was used.The mobile phase was composed of acetonitrile（A）-0.1%formic acid aqueous solution（B）in gradient elution mode：1-5 min,A-B（40∶60）;5-7 min A-B（40∶60-70∶30）;7-10 min A-B（70∶30）;10-11 min 100% A;11-12 min A-B（40∶60）with the flow rate of 0.3 mL·min^-1.The injection volume was 2μL and the column temperature was 30℃.ESI＋ion source and EIC mode were selected.RESULTS The linear relationship between the concentration and peak areas of the seven lignans were all linear（r〉0.999）.The average recoveries（n=9）were between 95.15% and 100.84%.CONCLUSION The method is rapid,sensitive,selective and suitable for the quantitative analysis of lignans in Shengmaiyin.
出处
《中国医院药学杂志》
CAS
北大核心
2017年第16期1590-1593,共4页
Chinese Journal of Hospital Pharmacy
基金
国家自然科学基金项目(编号:81403067)
黑龙江省自然科学基金项目(编号:C2016059)
关键词
生脉饮
木脂素
液质联用
含量测定
Shengmaiyin
lignans
UPLC-MS
content determination
