摘要
目的:探讨不同浓度的黄芩苷对胃癌SGC-7901细胞增殖的抑制作用,以及对pten和survivin基因的影响。方法:以不同浓度(10、20、40、80、160、320μmol/L)的黄芩苷处理胃癌SGC-7901细胞后,采用MTT法测定细胞活力;80、120、160μmol/L的黄芩苷处理胃癌SGC-7901细胞后,采用RT-q PCR法检测Toll样受体8(TLR8)、低氧诱导因子(HIF-1α)、血小板衍生生长因子β(PDGF-β)和第10号染色体上磷酸酶和张力白同源缺失性基因(pten)的表达,Western-blot法分别检测胃癌细胞TLR8、HIF-1α、PDGF-β和pten蛋白的表达。结果:不同浓度的黄芩苷均能抑制细胞增殖,RT-q PCR法检测显示TLR8、HIF-1α、PDGF-β基因表达下调,pten基因表达上调。结论:不同浓度的黄芩苷可抑制胃癌细胞增殖,并能上调pten和下调TLR8、HIF-1α、PDGF-βm RNA及蛋白的表达。
Objective： To discuss the inhibiting effects of baicalin with different concentrations on the proliferation of gastric cancer SGC-7901 cell, and its influence on the gene pten and survivin. Methods： Cellular activity was measured by adopting MTT method after dealing SGC-7901 cell with baiealin with different concentrations （10, 20, 40, 80, 160 and 320 μmol/L）; the expressions of TLR8, HIF-1α, PDGFβ and pten were detected by RT-qPCR method after dealing SGC-7901 cell with baicalin with different concentrations （80, 120 and 160 μmol/L）; the expressions of TLR8, HIF-1α , PDGFβ and pten were detected by Western-blot method respectively. Results： Baicalin with different concentrations could inhibit cellular proliferation, RT-qPCR method showed that the expressions of TLR8, HIF-1α and PDGFβ down regulated, while pten expression up regulated. Conclusion： Baicalin with different concentrations could inhibit gastric cancer cellular proliferation, and it could up regulate pten expressions and down regulate the expressions of TLR8, HIF-1α and PDGFβ mRNA and protein.
出处
《西部中医药》
2017年第8期12-16,共5页
Western Journal of Traditional Chinese Medicine
基金
2014甘肃省科技厅B类计划项目(编号148RJZA073)
2013甘肃省卫生行业管理项目(编号GWGL2013-40)
