摘要
建立了一种简便、快速、准确的喜树叶片中喜树碱含量的高效液相色谱分析方法 ;采用英国HPLCTechnology公司的TechsphereODS柱 (25cm×4.6mmID ,5μm) ,流动相乙腈 -水 (体积比4∶6) ,流速1.0mL·min -1,检测波长254nm ,柱温25℃ ,进样量10μL ;样品制备方法以61 % (φ)乙醇为溶剂 ,50℃下超声提取喜树叶粉10min;HPLC法测定喜树碱的含量 ;该法的RSD为2.5% (n=6) ,平均回收率为96 %。
A simple, rapid and sensitive method for the determination of camptothecin in leaves of Camptotheca acuminata Decne.was established. Camptothecin in the leaves was ultrasonically extracted with 61% alcohol at 50 ℃ for 10 min, and determined by high performance liquid chromatography. The camptothecin was separated on a Techsphere ODS column(25 cm×4.6 mm ID, 5 μm) at 25 ℃, using acetonitrile-water(4∶6 by volume) system with 1.0 mL/min as the mobile phase, and detected at 254 nm. Relative standard deviation of 2.5%(n=6) and average recovery of 96% for the method were found.
出处
《分析测试学报》
CAS
CSCD
北大核心
2002年第2期15-18,共4页
Journal of Instrumental Analysis
基金
国家自然科学基金资助项目 (30070086)
黑龙江省留学归国基金资助项目 (L00C009)
