摘要
目的:优选某中药抗癌复方中总黄酮的提取工艺。方法:采用正交试验法以乙醇浓度、提取时间、料液比设置三因素三水平正交实验,采用热回流提取法并浓缩成浸膏,运用紫外分光光度法测定总黄酮含量,以总黄酮的含量为指标确定最佳提取工艺条件。结果:总黄酮在0.0096~0.0576 mg/m L浓度范围内与吸光度呈良好线性关系,最佳工艺条件为A1B3C3,即乙醇浓度95%,提取时间120 min,料液比1︰12,总黄酮的含量最多为8.273%。结论:采用正交试验分析抗癌药方提取物的提取工艺合理可行。
Objectie： The extraction process of total flavonoids in traditional Chinese medicine anticancer compound. Methods： Orthogonal test was used to establish the three-factor three-level orthogonal experiment with ethanol concentration, extraction time and solid-liquid ratio. The total flavonoids were determined by UV spectrophotometry. The total flavonoids were determined by UV spectrophotometry. The optimum extraction conditions were determined as indicators. Results： The total flavonoids had a good linear relationship with absorbance in the range of 0.0096-0.0576 mg/mL. The optimum conditions were A IB3C3, ethanol concentration 95 %, extraction time 120 min, ratio of material to liquid 1 ： 12, total flavonoids Up to 8.273 %. Conclusion： It is reasonable and feasible to use orthogonal test to analyze the extraction process of anticancer drug extract.
出处
《广东化工》
CAS
2017年第15期56-57,72,共3页
Guangdong Chemical Industry
关键词
中药抗癌复方
总黄酮
含量测定
紫外分光光度法
正交试验
Chinese medicine compound
total flavonoids
determination of content
UV spectrophotometry
orthogonal test
