摘要
目的探讨β-arrestin 2的小分子干扰RNA(small interfering RNA,siRNA)对人乳腺癌Bcap37细胞增殖的影响。方法在脂质体Lipofectamine^(TM) 2000的介导下用β-arrestin 2 siRNA转染Bcap37细胞,同时设置空白对照组(不作任何处理)及阴性对照组(转染p GPU6/GFP/Neo SHNC)。转染后用实时荧光定量PCR(quantitative realtime polymerase chain reaction,qRT-PCR)及Western blot法检测Bcap37细胞β-arrestin 2基因及蛋白的表达情况,MTT法检测Bcap37细胞的增殖。结果转染96 h后β-arrestin 2 siRNA转染的Bcap37细胞中β-arrestin 2的基因及蛋白表达低于空白及阴性对照组(均P<0.05),并且转染96 h后β-arrestin 2 siRNA转染的细胞增殖速度低于空白及阴性对照组(均P<0.05)。结论β-arrestin 2 siRNA降低Bcap37细胞β-arrestin 2的表达并抑制细胞增殖。
Objective To investigate the effect of β-arrestin 2 small interfering RNA（ siRNA） on the proliferation of human breast cancer Bcap37 cells. Methods Bcap37 cells were transfected with β-arrestin 2 siRNA by lipofectamineTM2000. p GPU6/GFP/Neo SHNC transfected cells were used as the negative control and the non-transfected cells served as the blank control. The expression of β-arrestin 2 gene was analyzed by quantitative real-time polymerase chain reaction（ qRT-PCR） and Western blot. Cell proliferation was examined by MTT assay. Results The expression level of β-arrestin2 was significantly lower in the β-arrestin 2 siRNA group than that in the negative control or blank control groups（ both P〈0. 05）. The cell proliferation rate of β-arrestin 2 siRNA transfected cells was also significantly lower than that of two control groups（ both P〈0. 05）. Conclusion β-arrestin 2 siRNA can effectively down-regulate the expression of β-arrestin 2,and inhibit the proliferation of human breast cancer Bcap37 cells.
作者
胡美琴
高琨
Hu Meiqin Gao Kun(Department of Abdominal and Pelvic Oncology, Huangshi Central Hospital, Affiliated Hospital of Hubei Polytechnic University, Edong Healthcare Group, Huangshi 435000, Chin)
出处
《实用肿瘤杂志》
CAS
2017年第4期308-311,共4页
Journal of Practical Oncology
