摘要
目的运用结扎法建立肠系膜上静脉血栓形成大鼠模型,模拟该疾病的病理过程,为研究其发病机制和治疗方法提供基础准备。方法 96只雄性SD大鼠,随机分为三组,A组(假手术组)、B组(绞窄型组)和C组(单纯型组),每组大鼠32只。A组大鼠仅打开腹腔,不阻断血运,术后8、24、48、72 h分批处死大鼠;B组和C组大鼠分别运用结扎法建立肠系膜上静脉血栓形成绞窄型和单纯型模型,建模后8、24、48、72 h分批处死大鼠。HE染色观察大鼠肠道组织形态学变化并对损伤进行病理评分;ELISA法检测大鼠血清中肠脂肪酸结合蛋白(IFABP)和α-谷胱甘肽S转移酶(α-GST)水平。结果 HE染色和病理评分结果示,与A组大鼠相比,B组和C组大鼠肠道组织均出现不同程度的血液瘀滞和损伤,B组逐渐加重,C组逐渐减轻,且血液瘀滞和损伤程度与结扎范围呈正相关。血清ELISA法结果示,与A组大鼠相比,B组和C组大鼠血清中IFABP和α-GST水平均呈现不同程度的升高(P<0.05),且升高程度与结扎范围呈正相关。结论本研究运用结扎法成功建立了肠系膜上静脉血栓形成大鼠模型,简单易行,且手术成功率高,可在相关研究中使用。
Objective To establish a rat model of superior mesenteric vein thrombosis by vein ligation and to simulate the pathological process of the disease, and to provide the basis for studies of its pathogenesis and treatment. Methods Ninety-six SPF male SD rats were randomly divided into three groups： Group A （sham operation group） , group B （strangulation group） and group C （simple group）, 32 rats in each group. Rats in group A were only opened the abdominal cavity but not blocked the blood supply. The rats were sacrificed at 8, 24, 48 and 72 h after operation. The rats in groups B and C were subjected to establish the strangulation and simple models by superior mesenteric vein thrombosis, respectively, and were sacrificed at 8, 24, 48 and 72 h after modeling. Histological changes （H＆E staining） in the rat intestinal tissues were evaluated by a pathological scoring system. The levels of intestinal fatty acid binding protein （IFABP） and et-glutathione S-transferase （ct-GST） were detected by ELISA. Results The rat model of mesenteric vein thrombosis was successfully established, with a success rate of 100% （96/96）. The pathological analysis revealed that compared with the group A, different degrees of blood stasis and injuries were observed in the intestinal tissues of groups B and C, and the injury were gradually increased in the group B, while gradually reduced in the group C. The degrees of blood stasis and injury were positively correlated with tile scope of ligation. The result of EI：ISA showed that the serum levels of IFABP and α-GST of the rats in groups B and C were significantly higher than those in group A （ P 〈 0. 05 ） , and the degree of elevation was positively correlated with the scope of ligation. Conclusions In this study, the rat model of superior mesenterie vein thrombosis is successfully established by vein ligation. This model is simple and easy to operate with a high success rate, and can be used in related research.
作者
王阳
陈建立
陈俊卯
曹文斌
刘洋
张国志
WANG Yang CHEN Jian-li CHEN Jun-mao CAO Wen-bin LIU Yang ZHANG Guo-zhi(Department of General Surgery, North China University of Science and Technology Affiliated Hospital, Tangshan 063000, Chin)
出处
《中国比较医学杂志》
北大核心
2017年第7期64-69,共6页
Chinese Journal of Comparative Medicine
基金
河北省卫生厅医学科学研究重点课题(20100130)
关键词
肠系膜上静脉血栓形成
结扎法
大鼠模型
绞窄型
单纯型
Superior mesenleric vein thrombosis
Ligation
Rat model
Strangulation type
Simple type
