摘要
目的:观察动物24 h多次灌胃给予芒果苷的急性毒性。方法:将40只KM种小鼠随机分为给药组和对照组,6只Beagle犬随机分为给药组和对照组,均以最大耐受量(MTD)法灌胃给药进行毒性试验,给药后均观察14 d。结果:小鼠给药后,其外观、行为活动、饮食等均未见明显异常,体重增长正常,试验结束时全部存活,大体解剖未见肉眼可见病理变化;犬给药后,均出现一过性灰白色粪便,给药组在给药后次日出现TBIL明显升高,发生率为1/4,但观察结束后又恢复至给药水平,其余外观、血液生化指标等均无明显异常,大体解剖未见肉眼可明显异常。结论:小鼠灌胃给予芒果苷54 g/kg未产生明显毒性反应;犬灌胃给予芒果苷MTD>9.0 g/kg体重;有1/4例动物在给药次日TBIL明显升高,提示芒果苷有可能影响TBIL代谢,但能自行恢复。
Objective： To observe acute toxicity of Mangiferin for intragastric administration（ I. g） at multiple dose in mice and dogs. Methods： Forty KM mice and six Beagle dogs were randomly divided into treatment group and control group. Intragastric administered maxi malad administration dose（ MAD） based on approximate lethal dose（ ALD） and animals in both groups were observed for 14 days. Results： All mice were alive and no abnormality was found in appearance,behavior and diet. In addition,no pathological changes were found after dissection. Grey feces were found once in dogs and the experiment group showed increase of TBIL,with an occurrence rate of 25%,but it restored to normal level after administration. No abnormality was found in blood and biochemical indicators. No physiological abnormality was found after dissection. Conclusion： No toxicity symptoms was found after oral administered 54 g/kg of mangiferin. One of four dog＇s TBIL increased significantly after given mangiferin MTD〉 9. 0 g/kg,but it can restore to normal level.
出处
《世界中医药》
CAS
2017年第7期1668-1670,1678,共4页
World Chinese Medicine
基金
广西重点实验室建设项目(15-140-31)
广西农作物废弃物功能成分研究协同创新中心建设(CICAR2015-Z1)
