摘要
建立了高效液相色谱法测定芒果中芒果苷含量的方法,其中色谱柱为Waters C18(4.6 mm×150 mm,5μm);流动相为甲醇-0.04%磷酸(28∶72)为流速为1.0 mL.min-1,检测波长为258 nm,柱温为室温。测量结果为芒果苷线性范围0.008 25～0.016 5μg(r=0.9996);平均回收率为98.48%,RSD为2.65%。该方法简便、快速、线性关系良好,适合芒果中芒果苷的含量测定。
To establish the contents of mangiferin in mangifera indica by HPLC,the analysis of HPLC was carried out with Waters C18 column（4.6mm×150mm,5μm）.The mobile phase was CH_3OH-0.04%H_3PO_4（28∶72）with the flow-rate at 1.0 mL/min.The wave-length was 258nm with room temperature.Experiment results show that the liner range of mangiferin is 0.008 25～0.016 5μg（r=0.999 6）,the average recovery is 98.48% and RSD is 2.65%.The method is simple and fast,it has a good linear relationship and could be used for the contents detection of mangiferin in mangifera indica.
出处
《重庆理工大学学报（自然科学）》
CAS
2011年第10期23-25,48,共4页
Journal of Chongqing University of Technology：Natural Science
基金
科技部重大新药创制专项资助项目(2010ZX09401-306-2-23)
重庆市科委项目(2009GJF10023)
关键词
芒果
HPLC
芒果苷
mangifera indica
HPLC
mangiferin
