摘要
目的:探讨CCK-8法和LDH法检测CIK细胞活性的最佳条件。方法:经密度梯度法分离得到人外周血中单个核细胞,经细胞因子刺激培养14天收获CIK细胞,采用CCK-8法与LDH法检测CIK细胞对前列腺癌细胞毒活性。结果:CCK-8法的最佳检测时间为加入CCK-8试剂后3~4 h,适宜细胞数量范围为0.32×10~3~2.0×10~4个/孔。结论:CCK-8法较LDH法稳定性好,准确性好,操作简便,是一种优于LDH法的检测免疫细胞毒活性的方法。
Objective：To investigate the optimal conditions of CCK-8 and LDH assay for detecting CIK cell cytotoxic activity.Methods：Peripheral blood mononuclear cells（PBMCs）were isolated by Ficoll-Paque gradient centrifugation and CIK cells were amplified by cytokine.After culturing 14 days,the cytotoxicity of CIK cells to prostate cancer cells was detected by CCK-8 and LDH assay.Results：The best detection time of CCK-8 method was 3～4 h after adding CCK-8 reagent,and the suitable cell number range was 0.32×10^3～2.0×10^4 cells/hole.Conclusion：CCK-8 is a better method than LDH in stability,accuracy and operation to detect the cytotoxic activity of immune cells.
出处
《现代肿瘤医学》
CAS
2017年第16期2530-2533,共4页
Journal of Modern Oncology
基金
国家自然科学基金(编号:81402370)
广东省自然科学基金(编号:2015A030313829)
广东省科技项目产学研合作项目(编号:2015B090904007)
广东省科技厅粤港创新圈项目(编号:2016A050503009)
