摘要
目的探讨塞来昔布对大鼠颅脑创伤后环氧合酶(COX-2)和凋亡蛋白Caspase-9表达及运动功能的影响。方法实验分为对照组、假手术组、创伤组、治疗组,采用Marmarou方法建立大鼠闭合性颅脑创伤模型,实时荧光定量PCR(q PCR)检测COX-2和Caspase-9基因表达量,免疫组织化学染色法检测COX-2和Caspase-9蛋白的表达,神经功能损害评分(NSS)检测大鼠的运动功能。结果创伤组COX-2和Caspase-9基因和蛋白的表达明显高于其他3组(P<0.05),治疗组较创伤组能有效降低COX-2和Caspase-9的表达,但仍高于假手术组和正常组(P<0.05);与创伤组相比较,治疗组能有效改善大鼠的运动功能障碍(P<0.05),和对照组和假手术组比较差异具有统计学意义(P<0.05)。结论塞来昔布可通过对COX-2的特异性抑制作用,减少颅脑损伤后炎症反应,并进一步降低Caspase-9的表达,从而减少神经细胞的凋亡,并改善大鼠颅脑创伤后的运动功能障碍。
Aim To investigate the effects of celecoxib on cyclooxygenase-2( COX-2) and caspase-9 expression and motor function after traumatic brain injury in rats. Methods The rats were divided into control group,sham operation group,trauma group and treatment group. The model of closed craniocerebral trauma was established by Marmarou method,the gene expression of COX-2 and Caspase-9 was detected by real-time quantitative PCR( qPCR),the protein expressions of COX-2 and Caspase-9 were detected by immunohistochemical staining,and the motor function of the rats was evaluated by the neurological impairment score( NSS). Results The gene and protein expression of COX-2 and Caspase-9 in traumatic group was significantly higher than in other three groups( P < 0. 05),the expression of COX-2 and Caspase-9 in treatmentgroup was significantly lower than in traumatic group( P< 0. 05), but still higher than the sham operation group and the normal group( P < 0. 05); compared with the trauma group,the motor function of the treatment group could be effectively improve( P < 0. 05),but compared with the control group and the sham operation group,the difference was statistically significant( P < 0. 05). Conclusion Celecoxib can reduce the inflammatory response after craniocerebral injury by specific inhibition of COX-2,and further reduce the expression of Caspase-9,thereby reducing the apoptosis of nerve cells,and improving motor function after traumatic brain injury in rats.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2017年第6期873-877,共5页
Chinese Pharmacological Bulletin
基金
河北省自然科学基金项目(No C2004000689)
河北省博士基金项目(No 05547008D-4)
河北省科学技术与社会发展计划项目(No 04276135)
